Localization of adenosine-receptor subtype mRNA in rat outer medullary descending vasa recta by RT-PCR

被引:37
作者
Kreisberg, MS [1 ]
Silldorff, EP [1 ]
Pallone, TL [1 ]
机构
[1] PENN STATE UNIV, MILTON S HERSHEY MED CTR, DEPT MED, DIV NEPHROL, HERSHEY, PA 17033 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 1997年 / 272卷 / 03期
关键词
A(1) adenosine receptor; A(2a) adenosine receptor; A(2b) adenosine receptor; A(3) adenosine receptor; vasoregulation; microcirculation;
D O I
10.1152/ajpheart.1997.272.3.H1231
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Adenosine has a multitude of functions in the kidney, including vasoregulation of the renal vasculature. The actions of adenosine are mediated by its binding to specific receptors. Four adenosine-receptor subtypes have been cloned and sequenced, the A(1), A(2a), A(2b), and the A(3). In this study, the expression of individual adenosine-receptor subtype RNAs in outer medullary descending vasa recta (OMDVR) was investigated. Total RNA isolated from the outer medulla and microdissected, permeabilized OMDVR were subjected to reverse transcription-polymerase chain reaction (RT-PCR) with primers specific for each of the adenosine-receptor subtypes. Subtype-specific probes were used to verify the PCR products by Southern hybridization. Our studies, performed in triplicate on five different rats, indicate the presence of A(1), A(2a), and A(2b) adenosine-receptor subtype mRNAs. These products were not attributable to extraneous RNA contamination from other tissue sources, nor did they result from genomic DNA amplification. These data are consistent with pharmacological evaluations, favor A(1), A(2a), and A(2b) adenosine-receptor subtype expression in OMDVR, and support a role for adenosine in the regulation of medullary blood flow.
引用
收藏
页码:H1231 / H1238
页数:8
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