Functional analysis of spfD gene involved in DNA phosphorothioation in Pseudomonas fluorescens Pf0-1

被引：71

作者：

Yao, Fen

Xu, Tiegang

Zhou, Xiufen

Deng, Zixin ^{[1
]}

You, Delin

机构：

[1] Shanghai Jiao Tong Univ, Lab Microbial Metab, Shanghai 200030, Peoples R China

来源：

FEBS LETTERS | 2009年 / 583卷 / 04期

基金：

美国国家科学基金会;

关键词：

ATPase; DNA phosphorothioation; spfD; Pseudomonas fluorescens Pf0-1; ESCHERICHIA-COLI; STREPTOMYCES-LIVIDANS; MODIFICATION SITES; ABC TRANSPORTER; 4-THIOURIDINE; SULFUR; BIOSYNTHESIS; BACTERIA; SEQUENCE; PROTEIN;

D O I：

10.1016/j.febslet.2009.01.029

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

DNA phosphorothioation is widespread in many bacterial species. By homology analysis of the dnd gene cluster in Pseudomonas fluorescens Pf0-1, a spfBCDE gene cluster involved in DNA phosphorothioation was localized. Disruption of the spfD gene, a dndD homolog, caused the loss of the Dnd phenotype and demonstrated the involvement of spfD in DNA phosphorothioation in P. fluorescens Pf0-1. The ATPase activity of SpfD suggests that SpfD could hydrolyze ATP to provide the energy required in the DNA phosphorothioate modi. cation process. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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收藏

页码：729 / 733

页数：5

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