3-O-Acetyloleanolic acid exhibits anti-angiogenic effects and induces apoptosis in human umbilical vein endothelial cells

被引：8

作者：

Cui, En-Ji ^{[1
,2
]}

Hwang-Bo, Jeon ^{[1
,2
]}

Park, Jong-Hwa ^{[1
,2
]}

Baek, Nam-In ^{[2
]}

Kim, Jiyoung ^{[2
]}

Hong, Seong Gil ^{[3
]}

Chung, In Sik ^{[1
,2
]}

机构：

[1] Kyung Hee Univ, Dept Genet Engn, Yongin 446701, South Korea

[2] Kyung Hee Univ, Grad Sch Biotechnol, Yongin 446701, South Korea

[3] Erom Life Co Ltd, Seoul 135825, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2013年 / 35卷 / 11期

基金：

新加坡国家研究基金会;

关键词：

3-O-Acetyloleanolic acid; Apoptosis; Human umbilical vein endothelial cell; Matrigel plug assay; CANCER; TRITERPENOIDS; CARCINOMA; INHIBIT; GROWTH; SEEDS; BARK;

D O I：

10.1007/s10529-013-1266-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

3-O-Acetyloleanolic acid, a pentacyclic triterpenoid isolated from cowpea seeds, inhibited proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs) in a dose-dependent manner. HUVECs. The induced apoptosis was characterized by detection of cell surface annexin V and sub-G1 populations. The number of cells immunostained with annexin V-fluorescein isothiocyanate increased after treatment with 3-O-acetyloleanolic acid. The sub-G1 cell populations were also increased in treated HUVECs. 3-O-Acetyloleanolic acid induced activation of caspase 3, a critical mediator of apoptosis signaling. It also significantly inhibited angiogenesis in an in vivo Matrigel plug assay. 3-O-Acetyloleanolic acid thus exhibits anti-angiogenic effects and induces apoptosis in HUVECs and the results suggest that it has a potential use for suppression of the tumor growth stimulated by angiogenesis.

引用

页码：1807 / 1815

页数：9

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