Conjugatable water-soluble Pt(II) and Pd(II) porphyrin complexes: novel nano-and molecular probes for optical oxygen tension measurement in tissue engineering

被引：23

作者：

Giuntini, F. ^{[1
]}

Chauhan, V. M. ^{[2
]}

Aylott, J. W. ^{[2
]}

Rosser, G. A. ^{[3
]}

Athanasiadis, A. ^{[4
,5
]}

Beeby, A. ^{[3
]}

MacRobert, A. J. ^{[4
]}

Brown, R. A. ^{[5
]}

Boyle, R. W. ^{[1
]}

机构：

[1] Univ Hull, Dept Chem, Kingston Upon Hull HU6 7RX, N Humberside, England

[2] Univ Nottingham, Sch Pharm, Nottingham NG7 2RD, England

[3] Univ Durham, Dept Chem, Durham, England

[4] UCL, Natl Med Laser Ctr, London, England

[5] UCL, Inst Orthopaed & Musculoskeletal Sci, London, England

来源：

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES | 2014年 / 13卷 / 07期

基金：

英国工程与自然科学研究理事会;

关键词：

DISSOLVED-OXYGEN; POLYACRYLAMIDE NANOPARTICLES; INTRACELLULAR DELIVERY; PHOSPHORESCENT PROBES; MAMMALIAN-CELLS; REACTIVE OXYGEN; TURBID MEDIA; LIVE CELLS; FLUORESCENT; SENSORS;

D O I：

10.1039/c4pp00026a

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Measurement of oxygen tension in compressed collagen sheets was performed using matrix-embedded optical oxygen sensors based on platinum(II) and palladium(II) porphyrins supported on polyacrylamide nanoparticles. Bespoke, fully water-soluble, mono-functionalised Pt(II) and Pd(II) porphyrin complexes designed for conjugation under mild conditions were obtained using microwave-assisted metallation. The new sensors display a linear response (1/t vs. O-2) to varying oxygen tension over a biologically relevant range (7.0 x 10(-4) to 2.7 x 10(-1) mM) in aqueous solutions; a behaviour that is maintained following conjugation to polyacrylamide nanoparticles, and following embedding of the nanosensors in compressed collagen sheets, paving the way to innovative approaches for real-time resolution of oxygen gradients throughout 3D matrices useful for tissue regeneration.

引用

页码：1039 / 1051

页数：13

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