ChIP-seq: Using high-throughput sequencing to discover protein-DNA interactions

被引：378

作者：

Schmidt, Dominic ^{[1
,2
]}

Wilson, Michael D. ^{[2
]}

Spyrou, Christiana ^{[2
,3
]}

Brown, Gordon D. ^{[2
]}

Hadfield, James ^{[2
]}

Odom, Duncan T. ^{[1
,2
]}

机构：

[1] Hutchison MRC Res Ctr, Dept Oncol, Cambridge CB2 0XZ, England

[2] Li Ka Shing Ctr, Cambridge Res Inst, Canc Res UK, Cambridge CB2 0RE, England

[3] Univ Cambridge, Dept Pure Math & Math Stat, Stat Lab, Cambridge CB3 0WY, England

来源：

METHODS | 2009年 / 48卷 / 03期

基金：

欧洲研究理事会;

关键词：

ChIPseq; ChIP-seq; Chromatin immunoprecipitation; High-throughput sequencing; FACTOR-BINDING SITES; GENOME-WIDE ANALYSIS; CHROMATIN IMMUNOPRECIPITATION; IN-VIVO; FUNCTIONAL GENOMICS; TRANSCRIPTION; HUMAN-CHROMOSOME-21; TECHNOLOGIES; FORMALDEHYDE; LOCATION;

D O I：

10.1016/j.ymeth.2009.03.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatin immunoprecipitation (ChIP) allows specific protein-DNA interactions to be isolated. Combining ChIP with high-throughput sequencing reveals the DNA sequence involved in these interactions. Here, we describe how to perform ChIP-seq starting with whole tissues or cell lines, and ending with millions of short sequencing tags that can be aligned to the reference genome of the species under investigation. We also outline additional procedures to recover ChIP-chip libraries for ChIP-seq and discuss contemporary issues in data analysis. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：240 / 248

页数：9

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