Influence of nanohydroxyapatite surface properties on Staphylococcus epidermidis biofilm formation

被引:17
作者
Barros, J. [1 ,2 ,4 ]
Grenho, L. [1 ,2 ]
Manuel, C. M. [4 ,5 ]
Ferreira, C. [4 ]
Melo, L. [4 ]
Nunes, O. C. [4 ]
Monteiro, F. J. [1 ,2 ]
Ferraz, M. P. [1 ,3 ]
机构
[1] Univ Porto, INEB Inst Engn Biomed, P-4150180 Oporto, Portugal
[2] Univ Porto, FEUP Fac Med, Dept Met & Mat Engn, P-4150180 Oporto, Portugal
[3] Univ Fernando Pessoa, CEBIMED Ctr Estudos Biomed, Oporto, Portugal
[4] Univ Porto, Fac Engn, Dept Engn Quim, LEPAE, P-4150180 Oporto, Portugal
[5] ULP, Oporto, Portugal
关键词
nanohydroxyapatite; biofilm formation; Inoculum concentration; biomaterial surface area; biomaterial roughness; HYDROXYAPATITE CERAMICS; MEDICAL APPLICATIONS; BACTERIAL ADHERENCE; CELL-ADHESION; GLASS; BONE; SIZE; HYDROPHOBICITY; PROLIFERATION; MICROSPHERES;
D O I
10.1177/0885328213507300
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
Nanohydroxyapatite (nanoHA), due to its chemical properties, has appeared as an exceptionally promising bioceramic to be used as bone regeneration material. Staphylococcus epidermidis have emerged as major nosocomial pathogens associated with infections of implanted medical devices. In this work, the purpose was to study the influence of the nanoHA surface characteristics on S. epidermidis RP62A biofilm formation. Therefore, two different initial inoculum concentrations (C-i) were used in order to check if these would affect the biofilm formed on the nanoHA surfaces. Biofilm formation was followed by the enumeration of cultivable cells and by scanning electron microscopy. Surface topography, contact angle, total surface area and porosimetry of the biomaterials were studied and correlated with the biofilm data. The surface of nanoHA sintered at 830celcius (nanoHA830) showed to be more resistant to S. epidermidis attachment and accumulation than that of nanoHA sintered at 1000celcius (nanoHA1000). The biofilm formed on nanoHA830 presented differences in terms of structure, surface coverage and EPS production when compared to the one formed on nanoHA1000 surface. It was observed that topography and surface area of nanoHA surfaces had influence on the bacterial attachment and accumulation. C-i influenced bacteria attachment and accumulation on nanoHA surfaces over time. The choice of the initial inoculum concentration was relevant proving to have an effect on the extent of adherence thus being a critical point for human health if these materials are used in implantable devices. This study showed that the initial inoculum concentration and surface material properties determine the rate of microbial attachment to substrata and consequently are related to biofilm-associated infections in biomaterials.
引用
收藏
页码:1325 / 1335
页数:11
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