Development and evaluation of a DNA-barcoding approach for the rapid identification of nematodes

被引:124
作者
Bhadury, Punyasloke
Austen, Melanie C.
Bilton, David T.
Lambshead, P. John D.
Rogers, Alex D.
Smerdon, Gary R.
机构
[1] Plymouth Marine Lab, Plymouth PL1 3DH, Devon, England
[2] Univ Plymouth, Sch Biol Sci, Plymouth PL4 8AA, Devon, England
[3] Nat Hist Museum, Nematode Res Grp, Dept Zool, London SW7 5BD, England
[4] Zool Soc London, Inst Zool, London NW1 4RY, England
关键词
marine nematodes; identification; DNA barcoding; 18S rRNA; ecological survey;
D O I
10.3354/meps320001
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Free-living nematodes are abundant in all marine habitats, are highly diverse, and can be useful for monitoring anthropogenic impacts on the environment. Despite such attributes, nematodes are effectively ignored by many marine ecologists because of their time-consuming taxonomy. Nematode diagnostics has traditionally relied on detailed comparison of morphological characters which, given their abundance, is difficult and laborious, meaning that the biodiversity of the group is typically underestimated. Molecular methods such as DNA-barcoding offer potentially efficient alternative approaches to studying the biodiversity of marine nematode communities, allowing these organisms to be more effectively exploited in ecological surveys and environmental assessments. In this study, a number of nuclear and mitochondrial genomic regions were evaluated as potential diagnostic loci for marine nematode species identification. Of these, the 18S ribosomal RNA gene amplified most reliably from a range of taxa, and was therefore evaluated as a DNA barcode. In a comparison of molecular and morphological identifications, over 97 % of specimens sequenced were correctly assigned on the basis of a short stretch of 18S rRNA sequence (approximately 345 bp), making this a potentially useful marker for the rapid molecular assignment of unknown nematode species, and evaluation of nematode species richness during ecological surveys or environmental assessments. This study showed that a single marker approach based on amplification and sequencing may prove invaluable in the rapid identification of nematodes during ecological surveys and, indeed, other taxonomically challenging invertebrate taxa.
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页码:1 / 9
页数:9
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