A Field study to Estimate the Prevalence of Bovine African Trypanosomosis in Butaleja District, Uganda

被引：5

作者：

Jing, Zhang ^{[1
]}

Magona, Joseph W. ^{[2
,3
]}

Sakurai, Tatsuya ^{[1
]}

Thekisoe, Oriel M. M. ^{[1
]}

Otim, Charles P. ^{[3
]}

Sugimoto, Chihiro ^{[2
]}

Inoue, Noboru ^{[1
]}

机构：

[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan

[2] Hokkaido Univ, Res Ctr Zoonosis Control, Sapporo, Hokkaido 0600818, Japan

[3] NaLIRRI, Tororo, Uganda

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2009年 / 71卷 / 04期

关键词：

bovine; prevalence; trypanosome; Uganda; zoonotic parasite; RESISTANCE-ASSOCIATED GENE; SOUTHEAST UGANDA; CATTLE; BRUCEI; AMPLIFICATION; RHODESIENSE;

D O I：

10.1292/jvms.71.525

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Prevalence of bovine trypanosomosis was determined from a total of 203 blood samples collected from Butaleja district, eastern Uganda. All samples were examined by microhematocrit centrifuge test (MHC), PCR and ELISA. ELISA was performed in accordance with the OIE standard procedures using Trypanosoma brucei gambiense procyclic form crude antigens. PCR were utilized to identify the species and the subspecies of trypanosome. The overall prevalence of bovine African trypanosomosis was 8.9% by MHC, and 45.3% by the ELISA. Since Substantial number (12 out of 18) of MHC positive samples were negative in the PCR tests, we could not conclude the most epidemic trypanosome species in the studied area. Nevertheless, the PCR results Suggests that the most prevalent trypanosome was T. b. brucei (31/203), followed by T. congolense (6/203). In addition, only a few (3/203) mixed inflections of T. b. brucei and T. congolense was detected by the PCR. Results obtained from this Study indicates that bovine trypanosomosis is endemic in Butaleja district, Uganda.

引用

页码：525 / 527

页数：3

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