Protective effects of timosaponin B-II on high glucose-induced apoptosis in human umbilical vein endothelial cells

被引：51

作者：

Guo, Changrun ^{[1
]}

Li, Lu ^{[1
]}

Yang, Xiaolin ^{[2
]}

Meng, Zhaoqing ^{[1
]}

Li, Fei ^{[1
]}

Zhang, Chunfeng ^{[1
]}

Yang, Zhonglin ^{[1
]}

机构：

[1] China Pharmaceut Univ, State Key Lab Nat Med, Nanjing 210009, Jiangsu, Peoples R China

[2] Jinan Univ, Guangdong Prov Key Lab Pharmacodynam Constituents, Guangzhou 510632, Guangdong, Peoples R China

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2014年 / 37卷 / 01期

关键词：

Timosaponin B-II; Glucose; Endothelial cells; Reactive oxygen species; Caspase-3; Apoptosis; INDUCED OXIDATIVE STRESS; SAPONINS; RHIZOMA; DISEASE; KINASE;

D O I：

10.1016/j.etap.2013.11.009

中图分类号：

X [环境科学、安全科学];

学科分类号：

083001 [环境科学];

摘要：

This study was designed to investigate the action of timosaponin B-II, a main bioactive compound in Anemarrhena asphodeloides Bunge, on the prevention from high glucose-induced cytotoxicity and apoptosis in human umbilical vein endothelial cells (HUVECs) and the potential mechanisms involved. The results showed that compared with the normal control group, exposure of HUVECs to high glucose media for 72 h resulted in a significant increase in lactates dehydrogenise release, reactive oxygen species production, Caspase-3 activity and the percentage of apoptotic cells (p < 0.01). However, pretreatment with timosaponin B-II significantly increased the viability of HUVECs and decreased lactates dehydrogenise release, Caspase-3 activity and the apoptosis rate in a concentration-dependent manner (p < 0.05). In addition, timosaponin B-II notably decreased the amount of reactive oxygen species and malondialdehyde, as well as promoted glutathione peroxidase activity, endothelial nitric oxide synthase activity and nitric oxide release (p < 0.05). These results suggest that timosaponin B-II has the antiapoptotic effect in endothelial cells through inhibition of high glucose-induced oxidative stress and has the potential for preventing diabetic cardiovascular complications. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：37 / 44

页数：8

共 29 条

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