Use of soluble MHC class II peptide multimers to detect antigen-specific T cells in human disease

被引：8

作者：

Bill, JR

Kotzin, BL

机构：

[1] Univ Colorado, Hlth Sci Ctr, Div Clin Immunol B164, Dept Immunol, Denver, CO 80262 USA

[2] Univ Colorado, Hlth Sci Ctr, Dept Med, Denver, CO 80262 USA

[3] Natl Jewish Med & Res Ctr, Denver, CO USA

来源：

ARTHRITIS RESEARCH | 2002年 / 4卷 / 04期

关键词：

flow cytometry; MHC class II; MHC/peptide multimer; T-cell; T-cell receptor;

D O I：

10.1186/ar417

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Most techniques that identify antigen-specific T cells are dependent on the response of these cells to the relevant antigen in culture. Soluble multimers of MHC molecules, when occupied with the same peptide, will bind selectively to T cells specific for that MHC/peptide complex. Techniques to produce fluorescent MHC class II/peptide multimers have recently been developed. These reagents provide a method to facilitate detection and isolation of antigen-specific CD4(+) T cells and they represent a new research tool to study these cells in patients with immune-mediated diseases.

引用

收藏

页码：261 / 265

页数：5

相关论文

共 20 条

[1] Phenotypic analysis of antigen-specific T lymphocytes [J].

Altman, JD ;

Moss, PAH ;

Goulder, PJR ;

Barouch, DH ;

McHeyzerWilliams, MG ;

Bell, JI ;

McMichael, AJ ;

Davis, MM .

SCIENCE, 1996, 274 (5284) :94-96

[2] Initiation of signal transduction through the T cell receptor requires the peptide multivalent engagement of MHC ligands [J].

Boniface, JJ ;

Rabinowitz, JD ;

Wülfing, C ;

Hampl, J ;

Reich, Z ;

Altman, JD ;

Kantor, RM ;

Beeson, C ;

McConnell, HM ;

Davis, MM .

IMMUNITY, 1998, 9 (04) :459-466

[3] Cutting edge:: Detection of antigen-specific CD4+ T cells by HLA-DR1 oligomers is dependent on the T cell activation state [J].

Cameron, TO ;

Cochran, JR ;

Yassine-Diab, B ;

Sékaly, RP ;

Stern, LJ .

JOURNAL OF IMMUNOLOGY, 2001, 166 (02) :741-745

[4] A GENERAL-METHOD FOR FACILITATING HETERODIMERIC PAIRING BETWEEN 2 PROTEINS - APPLICATION TO EXPRESSION OF ALPHA-T-CELL AND BETA-T-CELL RECEPTOR EXTRACELLULAR SEGMENTS [J].

CHANG, HC ;

BAO, ZZ ;

YAO, Y ;

TSE, AGD ;

GOYARTS, EC ;

MADSEN, M ;

KAWASAKI, E ;

BRAUER, PP ;

SACCHETTINI, JC ;

NATHENSON, SG ;

REINHERZ, EL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (24) :11408-11412

[5] The relationship of MHC-peptide binding and T cell activation probed using chemically defined MHC class II oligomers [J].

Cochran, JR ;

Cameron, TO ;

Stern, LJ .

IMMUNITY, 2000, 12 (03) :241-250

[6] Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes [J].

Crawford, F ;

Kozono, H ;

White, J ;

Marrack, P ;

Kappler, J .

IMMUNITY, 1998, 8 (06) :675-682

[7] Use of soluble peptide-DR4 tetramers to detect synovial T cells specific for cartilage antigens in patients with rheumatoid arthritis [J].

Kotzin, BL ;

Falta, MT ;

Crawford, F ;

Rosloniec, EF ;

Bill, J ;

Marrack, P ;

Kappler, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (01) :291-296

[8] PRODUCTION OF SOLUBLE MHC CLASS-II PROTEINS WITH COVALENTLY BOUND SINGLE PEPTIDES [J].

KOZONO, H ;

WHITE, J ;

CLEMENTS, J ;

MARRACK, P ;

KAPPLER, J .

NATURE, 1994, 369 (6476) :151-154

[9] Human immunodeficiency virus type 1 envelope epitope-specific CD4+ T lymphocytes in simian/human immunodeficiency virus-infected and vaccinated rhesus monkeys detected using a peptide-major histocompatibility complex class II tetramer [J].

Kuroda, MJ ;

Schmitz, JE ;

Lekutis, C ;

Nickerson, CE ;

Lifton, MA ;

Franchini, G ;

Harouse, JM ;

Cheng-Mayer, C ;

Letvin, NL .

JOURNAL OF VIROLOGY, 2000, 74 (18) :8751-8756

[10] HLA-DQ tetramers identify epitope-specific T cells in peripheral blood of herpes simplex virus type 2-infected individuals: Direct detection of immunodominant antigen-responsive cells [J].

Kwok, WW ;

Liu, AW ;

Novak, EJ ;

Gebe, JA ;

Ettinger, RA ;

Nepom, GT ;

Reymond, SN ;

Koelle, DM .

JOURNAL OF IMMUNOLOGY, 2000, 164 (08) :4244-4249