A new spin on protein dynamics

被引：373

作者：

Columbus, L ^{[1
]}

Hubbell, WL

机构：

[1] Univ Calif Los Angeles, Jules Stein Eye Inst, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 2002年 / 27卷 / 06期

关键词：

D O I：

10.1016/S0968-0004(02)02095-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Site-directed spin labeling is a general method for investigating structure and conformational switching in soluble and membrane proteins. It will also be an important tool for exploring protein backbone dynamics. A semi-empirical analysis of nitroxide sidechain dynamics in spin-labeled proteins reveals contributions from fluctuations in backbone dihedral angles and rigid-body (collective) motions of alpha helices. Quantitative analysis of sidechain dynamics is sometimes possible, and contributions from backbone modes can be expressed in terms of relative order parameters and rates. Dynamic sequences identified by site-directed spin labeling correlate with functional domains, and so nitroxide scanning could provide an efficient strategy for identifying such domains in high-molecular weight proteins, supramolecular complexes and membrane proteins.

引用

页码：288 / 295

页数：8

共 58 条

[1] Structural features and light-dependent changes in the sequence 306-322 extending from helix VII to the palmitoylation sites in rhodopsin: A site-directed spin-labeling study
Altenbach, C
Cai, KW
Khorana, HG
Hubbell, WL
[J]. BIOCHEMISTRY, 1999, 38 (25) : 7931 - 7937
[2] Structural features and light-dependent changes in the cytoplasmic interhelical E-F loop region of rhodopsin: A site-directed spin-labeling study
Altenbach, C
Yang, K
Farrens, DL
Farahbakhsh, ZT
Khorana, HG
Hubbell, WL
[J]. BIOCHEMISTRY, 1996, 35 (38) : 12470 - 12478
[3] Structural features and light-dependent changes in the sequence 59-75 connecting helices I and II in rhodopsin: A site-directed spin-labeling study
Altenbach, C
Klein-Seetharaman, J
Hwa, J
Khorana, HG
Hubbell, WL
[J]. BIOCHEMISTRY, 1999, 38 (25) : 7945 - 7949
[4] TRANSMEMBRANE PROTEIN-STRUCTURE - SPIN LABELING OF BACTERIORHODOPSIN MUTANTS
ALTENBACH, C
MARTI, T
KHORANA, HG
HUBBELL, WL
[J]. SCIENCE, 1990, 248 (4959) : 1088 - 1092
[5] A multifrequency electron spin resonance study of T4 lysozyme dynamics
Barnes, JP
Liang, ZC
Mchaourab, HS
Freed, JH
Hubbell, WL
[J]. BIOPHYSICAL JOURNAL, 1999, 76 (06) : 3298 - 3306
[6] Berliner L. J., 1976, SPIN LABELING THEORY, DOI [DOI 10.1016/B978-0-12-092350-2.50008-4, 10.1016/B978-0-12-092350-2.50008-4]
[7] Temperature dependence of intramolecular dynamics of the basic leucine zipper of GCN4: Implications for the entropy of association with DNA
Bracken, C
Carr, PA
Cavanagh, J
Palmer, AG
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1999, 285 (05) : 2133 - 2146
[8] Nonlinear-least-squares analysis of slow-motion EPR spectra in one and two dimensions using a modified Levenberg-Marquardt algorithm
Budil, DE
Lee, S
Saxena, S
Freed, JH
[J]. JOURNAL OF MAGNETIC RESONANCE SERIES A, 1996, 120 (02) : 155 - 189
[9] Molecular motion of spin labeled side chains in α-helices:: Analysis by variation of side chain structure
Columbus, L
Kálai, T
Jekö, J
Hideg, K
Hubbell, WL
[J]. BIOCHEMISTRY, 2001, 40 (13) : 3828 - 3846
[10] SITE-SPECIFIC INCORPORATION OF BIOPHYSICAL PROBES INTO PROTEINS
CORNISH, VW
BENSON, DR
ALTENBACH, CA
HIDEG, K
HUBBELL, WL
SCHULTZ, PG
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (08) : 2910 - 2914

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