Isolation and characterization of a calendic acid producing (8,11)-linoleoyl desaturase

被引：16

作者：

Fritsche, K

Hornung, E

Peitzsch, N

Renz, A

Feussner, I

机构：

[1] Leibniz Inst Pflanzenbiochem, D-06120 Halle, Germany

[2] BASF AG, ZHP TA30, Pflanzenbiotechnol, D-67056 Ludwigshafen, Germany

来源：

FEBS LETTERS | 1999年 / 462卷 / 03期

关键词：

acyl-group desaturase; seed oil; Calendula officinalis; Saccharomyces cerevisiae;

D O I：

10.1016/S0014-5793(99)01541-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

For the biosynthesis of calendic acid a (8,11)-linoleoyl desaturase activity has been proposed. To isolate this desaturase, PCR-based cloning was used. The open reading frame of the isolated full-length cDNA is a 1131 bp sequence encoding a protein of 377 amino acids, For functional identification the cDNA was expressed in Saccharomyces cerevisiae, and formation of calendic acid was analyzed by RP-HPLC. The expression of the heterologous enzyme resulted in a significant amount of calendic acid presumably esterified within phospholipids, The results presented here identify a gene encoding a new type of (1,4)-acyl lipid desaturase. (C) 1999 Federation of European Biochemical Societies.

引用

页码：249 / 253

页数：5