Efficiency of four secondary enrichment protocols in differentiation and isolation of Listeria spp. and Listeria monocytogenes from smoked fish processing chains

被引:16
作者
Duarte, G
Vaz-Velho, M
Capell, C
Gibbs, P
机构
[1] Univ Catolica Portuguesa, Escola Super Biotecnol, P-4200 Porto, Portugal
[2] Politecn Viana Castelo, Escola Super Tecnol & Gestao, Porto, Portugal
[3] Leatherhead Food Res Assoc, Surrey, England
关键词
cold-smoked fish; Listeria; Listeria monocytogenes; secondary selective enrichments; conventional cultural and bactometer; capacitance-based detection methods;
D O I
10.1016/S0168-1605(99)00121-X
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Four secondary enrichment protocols (conventional methods: UVM II, Fraser 24 h and Fraser 48 h: Impedimetric method: Listeria electrical detection medium) were studied for their ability to isolate Listeria spp. and Listeria monocytogenes from fish and environmental samples collected along the processing chain of cold-smoked fish. From all methods, Listeria spp. and L. monocytogenes were respectively present in 56 and 34 of 315 samples analysed. Fraser broth incubated for 48 h gave the fewest false negative Listeria spp. results [4/56; (7.1%)], but concurrently only 15/34 (44.1%) samples were correctly identified as containing L. monocytogenes. Listeria electrical detection (LED) medium detected only 36/56 (64.3%) Listeria spp. positive samples. Despite this lower isolation rate, LED identified 20/34 (58.8%) L. monocytogenes positive samples correctly and gave fewer false positive results. The overall conclusion was that more than one isolation method is needed to accurately estimate L. monocytogenes contamination rates. (C) 1999 Elsevier Science BN. All rights reserved.
引用
收藏
页码:163 / 168
页数:6
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