Neuropeptide FF reverses the effect of mu-opioid on Ca2+ channels in rat spinal ganglion neurones

被引：39

作者：

Rebeyrolles, S ^{[1
]}

Zajac, JM ^{[1
]}

Roumy, M ^{[1
]}

机构：

[1] INST PHARMACOL & BIOL STRUCT,F-31077 TOULOUSE,FRANCE

来源：

NEUROREPORT | 1996年 / 7卷 / 18期

关键词：

intracellular calcium; neuropeptide FF; mu-opioid agonist; spinal ganglion neurones;

D O I：

10.1097/00001756-199611250-00036

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

SINGLE neurones isolated from spinal ganglia of young rats were loaded with Fluo3, a fluorescent indicator of intracellular Ca2+ concentration. In 27 of 47 neurones the depolarization-evoked rise in fluorescence was reduced by 47.8 +/- 3.3% by prior perfusion with DAGO (1 mu M, 30s) a mu-opioid agonist. In 12 neurones an analogue of neuropeptide FF ((1DMe)Y8Fa, 10 nM, 30 min) did not affect the Ca2+ response to depolarization. (1DMe)Y8Fa reversed the effect of DAGO by 63 +/- 8% in seven of these 12 neurones. We conclude that stimulation of neuropeptide FF receptors antagonizes mu-opioid modulation of Ca2+ channels at the cellular level.

引用

页码：2979 / 2981

页数：3

共 14 条

[1] AUTORADIOGRAPHIC DISTRIBUTION OF RECEPTORS TO FLFQPQRFAMIDE, A MORPHINE-MODULATING PEPTIDE, IN RAT CENTRAL-NERVOUS-SYSTEM [J].

ALLARD, M ;

ZAJAC, JM ;

SIMONNET, G .

NEUROSCIENCE, 1992, 49 (01) :101-116

[2] CHARACTERIZATION OF RAT SPINAL-CORD RECEPTORS TO FLFQPQRFAMIDE, A MAMMALIAN MORPHINE MODULATING PEPTIDE - A BINDING STUDY [J].

ALLARD, M ;

GEOFFRE, S ;

LEGENDRE, P ;

VINCENT, JD ;

SIMONNET, G .

BRAIN RESEARCH, 1989, 500 (1-2) :169-176

[3] ANALOGS OF F8FAMIDE RESISTANT TO DEGRADATION, WITH HIGH-AFFINITY AND INVIVO EFFECTS [J].

GICQUEL, S ;

MAZARGUIL, H ;

ALLARD, M ;

SIMONNET, G ;

ZAJAC, JM .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1992, 222 (01) :61-67

[4] Presence of neuropeptide FF receptors on primary afferent fibres of the rat spinal cord [J].

Gouarderes, C ;

Kar, S ;

Zajac, JM .

NEUROSCIENCE, 1996, 74 (01) :21-27

[5] ANTINOCICEPTIVE EFFECTS OF INTRATHECALLY ADMINISTERED F8FAMIDE AND FMRFAMIDE IN THE RAT [J].

GOUARDERES, C ;

SUTAK, M ;

ZAJAC, JM ;

JHAMANDAS, K .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1993, 237 (01) :73-81

[6] INHIBITORY INFLUENCES OF MAMMALIAN FMRFAMIDE (PHE-MET-ARG-PHE-AMIDE)-RELATED PEPTIDES ON NOCICEPTION AND MORPHINE-INDUCED AND STRESS-INDUCED ANALGESIA IN MICE [J].

KAVALIERS, M .

NEUROSCIENCE LETTERS, 1990, 115 (2-3) :307-312

[7]

MOISES HC, 1994, J NEUROSCI, V14, P5903

[8] Effects of neuropeptide FF on intracellular Ca2+ in mouse spinal ganglion neurons [J].

Roumy, M ;

Zajac, JM .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1996, 306 (1-3) :291-295

[9]

RUSIN KI, 1995, J NEUROSCI, V15, P4315

[10] ACTIVATION OF MU-OPIOID RECEPTORS INHIBITS TRANSIENT HIGH-THRESHOLD AND LOW-THRESHOLD CA2+ CURRENTS, BUT SPARES A SUSTAINED CURRENT [J].

SCHROEDER, JE ;

FISCHBACH, PS ;

ZHENG, D ;

MCCLESKEY, EW .

NEURON, 1991, 6 (01) :13-20

← 1 2 →