Gabaergic-benzodiazepine system is involved in the crotoxin-induced anxiogenic effect

被引:26
作者
Moreira, EG
Nascimento, N
Rogero, JR
Vassilieff, VS [1 ]
机构
[1] Univ Estadual Paulista, IBB, Ctr Assistencia Toxicol, BR-18618000 Botucatu, SP, Brazil
[2] CNEN, IPEN, Supervisao Radiobiol Coordenaria Bioengn, BR-05508900 Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
Crotalus durissus terrificus venom; crotoxin; anxiogenic compound; anxiety; exploration; open field; holeboard; social interaction; rotarod;
D O I
10.1016/S0091-3057(99)00177-X
中图分类号
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要
The behavioral effects of crotoxin (CTX), the major component of Crotalus durissus terrificus venom, were studied in rats submitted to the open field, holeboard, and social interaction tests. CTX (100, 250, and 500 mu g/kg, IP) was administered 2 h before the tests. In the open field, CTX reduced ambulation (250 mu g/kg) and rearing (250 and 500 mu g/kg) and increased grooming (100 and 250 mu g/kg) and freezing (250 mu g/kg). In the holeboard and social interaction, all the CTX doses evaluated decreased, respectively, head dip and head dipping, and social interaction time. The CTX-induced behavioral alterations could be attributed to its neuromuscular transmission blockade, but this possibility was ruled out because CTX (250 and 500 mu g/kg, IP, 2 h before the rotarod test) was unable to modify the rotarod performance of rats. The involvement of the benzodiazepine receptor in the CTX-induced behavioral alterations was investigated through the pretreatment (30 min before the tests, IP) of the animals with diazepam (1.2 mg/kg), or flumazenil (4 and 10 mg/kg). Both diazepam and flumazenil antagonized the CTX induced behavioral alterations in the open field, holeboard, and social interaction tests. This study demonstrated that: (1) CTX is an anxiogenic compound; and (2) the gabaergic-benzodiazepine system may play a role in the CTX-induced anxiogenic effect. (C) 1999 Elsevier Science Inc.
引用
收藏
页码:7 / 13
页数:7
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