High-performance liquid chromatographic analysis of phenobarbital and phenobarbital metabolites in human urine

被引：12

作者：

Paibir, SG ^{[1
]}

Soine, WH ^{[1
]}

机构：

[1] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,SCH PHARM,DEPT MED CHEM,RICHMOND,VA 23298

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 691卷 / 01期

关键词：

phenobarbital; p-hydroxyphenobarbital; phenobarbital N-glucosides; phenobarbital N-glucuronides;

D O I：

10.1016/S0378-4347(96)00437-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A HPLC assay using UV detection and post-column alkalinization was developed to quantify possible urinary excretion products of phenobarbital in human urine. After filtration the urine was injected directly onto the HPLC column for analysis of phenobarbital, p-hydroxyphenobarbital, phenobarbital N-glucosides and phenobarbital N-glucuronides. The accuracy and precision of the assay were within +/-15% and the limit of detection (LOD) was 1 mu M, suitable for pharmacokinetic studies. Phenobarbital was administered orally to five male subjects and urine was collected for a period of 96-108 h. Phenobarbital, p-hydroxyphenobarbital, and phenobarbital N-glucosides were detected and quantified in the urine of all five subjects. The phenobarbital N-glucuronides were not detected in the urine. This assay provides a rapid method with improved selectivity to analyze urine for phenobarbital and its metabolites.

引用

页码：111 / 117

页数：7

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