Diurnal variation in orexin A immunoreactivity and prepro-orexin mRNA in the rat central nervous system

被引：155

作者：

Taheri, S ^{[1
]}

Sunter, D ^{[1
]}

Dakin, C ^{[1
]}

Moyes, S ^{[1
]}

Seal, L ^{[1
]}

Gardiner, J ^{[1
]}

Rossi, M ^{[1
]}

Ghatei, M ^{[1
]}

Bloom, S ^{[1
]}

机构：

[1] Hammersmith Hosp, Imperial Coll Sch Med, Div Invest Sci, Endocrine Unit, London W12 0NN, England

来源：

NEUROSCIENCE LETTERS | 2000年 / 279卷 / 02期

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

diurnal variation; orexin A; prepro-orexin mRNA; radioimmunoassay; northern blot analysis; sleep; arousal;

D O I：

10.1016/S0304-3940(99)00955-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Orexins are a family of neuropeptides originally believed to be im portent mediators of food intake. The wide distribution of orexins and their receptors, however, has suggested other regulatory functions for these peptides including involvement in sleep and arousal mechanisms. In this study, we have demonstrated diurnal variation in orexin A immunoreactivity in the pens, from where locus coeruleus noradrenergic neurones innervate other brain areas to stimulate arousal, and in the preoptic/anterior hypothalamic region, an area implicated in the regulation of sleep and circadian rhythms. Orexin A immunoreactivity decreased by 50% in the preoptic/anterior hypothalamus from 09:00 to 21:00 h (P < 0.0001), whilst in the pens, it increased by over 30% from 09:00 to 01:00 h (P = 0.02). Prepro-orexin mRNA also displayed diurnal variation. This further suggests that orexins are involved in the regulation of the sleep/wake cycle. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：109 / 112

页数：4

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