Comparison of quantitative high performance thin layer chromatography and the high performance liquid chromatography of parabens

被引：47

作者：

Thomassin, M ^{[1
]}

Cavalli, E ^{[1
]}

Guillaume, Y ^{[1
]}

Guinchard, C ^{[1
]}

机构：

[1] FAC MED & PHARM,CHIM ANALYT LAB,F-25030 BESANCON,FRANCE

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1997年 / 15卷 / 06期

关键词：

p-hydroxybenzoic esters; high performance thin layer chromatography; high performance liquid chromatography; rapid quantitative analysis method;

D O I：

10.1016/S0731-7085(96)01914-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method is described for the densitometric determination of the p-hydroxybenzoic esters and p-hydroxybenzoic acid in mixtures or in drugs. This method is compared with the one used in high performance liquid chromatography (HPLC). The calibration curves were linear in interval 0.250-3.60 mu mol ml(-1) per 200 nl per spot. The limit of detection and the relative standard deviation (RSD) are higher than in HPLC (RSD is 6% in HPTLC, 3% in HPLC; limit of detection about 40 pmol in HPTLC and 25 pmol in HPLC) but HPTLC quantitative determination of parabens in drugs is faster.

引用

页码：831 / 838

页数：8

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