Inducible expression of p21(WAF-1/CIP-1/SDI-1) from a promoter conversion retroviral vector

被引：17

作者：

Mrochen, S

Klein, D

Nikol, S

Smith, JR

Salmons, B

Gunzburg, WH

机构：

[1] UNIV VET SCI,INST VIROL,A-1210 VIENNA,AUSTRIA

[2] GSF,NAT RES CTR ENVIRONM & HLTH,INST MOL VIROL,OBERSCHLEISSHEIM,GERMANY

[3] UNIV MUNICH,KLINIKUM GROSSHADERN,MED KLIN 1,D-8000 MUNICH,GERMANY

[4] BAVARIAN NORD RES INST,MUNICH,GERMANY

[5] BAYLOR COLL MED,DEPT CELL BIOL,HOUSTON,TX 77030

[6] BAYLOR COLL MED,DEPT MED,HOUSTON,TX 77030

[7] BAYLOR COLL MED,DIV MOL VIROL,HUFFINGTON CTR AGING,HOUSTON,TX

来源：

JOURNAL OF MOLECULAR MEDICINE-JMM | 1997年 / 75卷 / 11-12期

关键词：

retroviral vector; conditional expression; cell cycle control; mouse mammary tumor virus long terminal repeat; WAF-1/CIP-1/SDI-1;

D O I：

10.1007/s001090050171

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Constitutive, high-level expression of the potentially therapeutic WAF-1/CIP-1/SDI-1 gene is incompatible with cell growth. A promoter conversion retroviral vector carrying the WAF-1/CIP-1/SDI-1 gene under the transcriptional control of the glucocorticoid inducible promoter of mouse mammary tumor virus was used to infect human bladder carcinoma or feline kidney cells. Reduced cell growth due to a greater proportion of cells being in the G(0)/G(1) phase of the cell cycle was observed when WAF-1/CIP-1/SDI-1 expression was activated by addition of glucocorticoid hormone. This system demonstrates the potential long-term therapeutic use of WAF-1/CIP-1/SDI-1 delivered by retroviral vectors for inhibiting the growth of rapidly proliferating cells, Moreover. the conditional expression of genes such as WAF-1/CIP-1/SDI-1 from such retroviral vectors may facilitate analysis of their function.

引用

页码：820 / 828

页数：9

共 24 条

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