Profiling analysis of oligosaccharides in antibody pharmaceuticals by capillary electrophoresis

被引：62

作者：

Kamoda, S

Nomura, C

Kinoshita, M

Nishiura, S

Ishikawa, R

Kakehi, K

Kawasaki, N

Hayakawa, T

机构：

[1] Kinki Univ, Fac Pharmaceut Sci, Higashiosaka, Osaka 5778502, Japan

[2] KIRIN BREWRY Co Ltd, Takasaki, Gumma 3700013, Japan

[3] Kinki Univ, Nara Hosp, Ikoma 6300293, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1050卷 / 02期

关键词：

oligosaccharide; capillary electrophoresis; 3-aminobenzoic acid; 8-aminopyrene-1,3,6-trisulfonate; antibody pharmaceutical;

D O I：

10.1016/j.chroma.2004.08.049

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Carbohydrate chains in glycoprotein pharmaceuticals have important roles for the expression of their biological activities. Therefore, development of an assessment method for the carbohydrate chains is an important parameter for quality control of glycoprotein pharmaceuticals such as newly developed therapeutic antibodies. In this report, we applied capillary electrophoresis with laser-induced fluorescence detection to the analysis of carbohydrate chains after releasing with glycoamidase followed by derivatization with 3-aminobenzoic acid. We found that four major oligosaccharides present in antibody pharmaceuticals were successfully separated with good resolution. The present method showed good precision in both migration times and relative peak areas, and gave comparable accuracy with that using a derivatization method with 8-aminopyrene-1,3,6-trisulfonate. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：211 / 216

页数：6

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