Rapid detection and identification of bacterial pathogens using novel molecular technologies: Infection control and beyond

被引：51

作者：

Tenover, Fred C. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Div Healthcare Qual Promot G08, Atlanta, GA 30333 USA

来源：

CLINICAL INFECTIOUS DISEASES | 2007年 / 44卷 / 03期

关键词：

D O I：

10.1086/510684

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The rapid detection and reporting of antimicrobial- resistant pathogens, such as methicillin- resistant Staphylococcus aureus, vancomycin- resistant enterococci, and multidrug- resistant Mycobacterium tuberculosis, is a challenge for the clinical microbiology laboratory. Molecular- based diagnostic tests can provide data on the presence of methicillin- resistant S. aureus in the nares in similar to 1 h, whereas testing for the vanA and vanB resistance genes in enterococci isolated from perirectal samples can be completed in similar to 4 h. Novel pyrosequencing assays can provide data regarding the presence of multidrug- resistant M. tuberculosis directly from positive mycobacterial broth cultures in < 1 day. These data can assist physicians in both therapeutic and infection control decisions.

引用

页码：418 / 423

页数：6

共 25 条

[1] Pyrosequencing: History, biochemistry and future
Ahmadian, A
Ehn, M
Hober, S
[J]. CLINICA CHIMICA ACTA, 2006, 363 (1-2) : 83 - 94
[2] Cost savings with implementation of PNA FISH testing for identification of Candida albicans in blood cultures
Alexander, BD
Ashley, ED
Reller, LB
Reed, SD
[J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2006, 54 (04) : 277 - 282
[3] [Anonymous], MMWR MORB MORTAL WKL
[4] Single-nucleotide polymorphism-based differentiation and drug resistance detection in Mycobacterium tuberculosis from isolates or directly from sputum
Arnold, C
Westland, L
Mowat, G
Underwood, A
Magee, J
Gharbia, S
[J]. CLINICAL MICROBIOLOGY AND INFECTION, 2005, 11 (02) : 122 - 130
[5] Molecular characterization of vanB elements in naturally occurring gut anaerobes
Ballard, SA
Pertile, KK
Lim, M
Johnson, PDR
Grayson, ML
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (05) : 1688 - 1694
[6] Comparison of three PCR primer sets for identification of vanB gene carriage in feces and correlation with carriage of vancomycin-resistant enterococci:: Interference by vanB-containing anaerobic bacilli
Ballard, SA
Grabsch, EA
Johnson, PDR
Grayson, ML
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (01) : 77 - 81
[7] MULTIDRUG-RESISTANT TUBERCULOSIS
DOOLEY, SW
JARVIS, WR
MARTONE, WJ
SNIDER, DE
[J]. ANNALS OF INTERNAL MEDICINE, 1992, 117 (03) : 257 - 259
[8] Real-time PCR in clinical microbiology: Applications for a routine laboratory testing
Espy, MJ
Uhl, JR
Sloan, LM
Buckwalter, SP
Jones, MF
Vetter, EA
Yao, JDC
Wengenack, NL
Rosenblatt, JE
Cockerill, FR
Smith, TF
[J]. CLINICAL MICROBIOLOGY REVIEWS, 2006, 19 (01) : 165 - +
[9] Impact of rapid in situ hybridization testing on coagulase-negative staphylococci positive blood cultures
Forrest, Graerne N.
Mehta, Sanjay
Weekes, Elizabeth
Lincalis, Durry P.
Johnson, Jennifer K.
Venezia, Richard A.
[J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2006, 58 (01) : 154 - 158
[10] New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci
Huletsky, A
Giroux, R
Rossbach, V
Gagnon, M
Vaillancourt, M
Bernier, M
Gagnon, F
Truchon, K
Bastien, M
Picard, FJ
van Belkum, A
Ouellette, M
Roy, PH
Bergeron, MG
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (05) : 1875 - 1884

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