Impact of cryopreservation on B cell chronic lymphocytic leukaemia phenotype

被引：15

作者：

Deneys, V ^{[1
]}

Thiry, V ^{[1
]}

Hougardy, N ^{[1
]}

Mazzon, AM ^{[1
]}

Leveugle, P ^{[1
]}

De Bruyère, M ^{[1
]}

机构：

[1] Univ Catholique Louvain, Univ Hosp, Immunohaematol Lab, B-1200 Brussels, Belgium

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1999年 / 228卷 / 1-2期

关键词：

chronic lymphocytic leukaemia; immunophenotype; cryopreservation; flow cytometry; cluster of differentiation;

D O I：

10.1016/S0022-1759(99)00089-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background and objectives: Freezing is a practical approach for cell preservation for retrospective studies. The aim of this work was to check the cryopreservation impact on B cell chronic lymphocytic leukaemia phenotype. Material and methods: Blood samples from 15 CLL patients were analyzed freshly and after freezing at -196 degrees C, without separation, and thawing. Results were compared by Student's paired t-test. Results: The phenotype of fresh CLL cells was as follows: CD19 +, CD5 +, faint CD20, CD23 +/-, weak CD22 and sig, CD37 +, HLA-DR +, FMC7 -. After cryopreservation, the percentage of CD5 and CD23 positive cells decreased, whereas HLA-DR positive cells increased moderately. The CLL Matures's score was modified in 6 cases out of 15 (40%). Conclusion: Cryopreservation modifies B cell chronic lymphocytic leukaemia phenotype, by decreasing CD5 and CD23 expression. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：13 / 21

页数：9

共 21 条

[1]

[Anonymous], ATLAS LYMPHOID HYPER

[2]

BARCLAY AN, 1997, LEUCOCYTE ANTIGEN

[3] Evaluation of methods for preparing and thawing cryopreserved CD34+ and CD34- cell lines for use as reagents in flow cytometry of hematopoietic progenitor cells [J].

Bowman, CA ;

Yu, M ;

CottlerFox, M .

TRANSFUSION, 1996, 36 (11-12) :985-988

[4] EXPRESSION OF IMMUNOLOGICAL MARKERS ON LEUKEMIC-CELLS BEFORE AND AFTER CRYOPRESERVATION AND THAWING [J].

CAMPOS, L ;

GUYOTAT, D ;

LARESE, A ;

ARCHIMBAUD, E ;

MAZET, L ;

EHRSAM, A ;

FIERE, D .

CRYOBIOLOGY, 1988, 25 (01) :18-22

[5]

Fiebig EW, 1997, CYTOMETRY, V29, P340, DOI 10.1002/(SICI)1097-0320(19971201)29:4<340::AID-CYTO11>3.0.CO

[6]

2-U

[7] QUALITY-CONTROL IN CLINICAL FLOW-CYTOMETRY [J].

HARVATH, L .

PATHOLOGY AND IMMUNOPATHOLOGY RESEARCH, 1988, 7 (05) :338-344

[8] PERIPHERAL-BLOOD CELL PREPARATION INFLUENCES THE LEVEL OF EXPRESSION OF LEUKOCYTE CELL-SURFACE MARKERS AS ASSESSED WITH QUANTITATIVE MULTICOLOR FLOW-CYTOMETRY [J].

ISLAM, D ;

LINDBERG, AA ;

CHRISTENSSON, B .

CYTOMETRY, 1995, 22 (02) :128-134

[9]

KAROW A M JR, 1965, Cryobiology, V2, P99, DOI 10.1016/S0011-2240(65)80094-3

[10]

Keeney M, 1998, CYTOMETRY, V34, P280, DOI 10.1002/(SICI)1097-0320(19981215)34:6<280::AID-CYTO6>3.0.CO

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