DNA adsorption to laterally structured charged lipid membranes

被引:48
作者
Clausen-Schaumann, H [1 ]
Gaub, HE [1 ]
机构
[1] LMU Munchen, Lehrstuhl Angew Phys, D-80799 Munich, Germany
关键词
D O I
10.1021/la990273b
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A macroscopic surface property, such as a surface's affinity for a certain polymer, may depend significantly on surface parameters on the nanoscopic length scale, such as the size of areas with positive and negative surface charge. In this study, polyelectrolyte adsorption to laterally structured charged surfaces was investigated as a function of the size of the charged surface areas. Polydeoxyribonucleic acid (DNA) was adsorbed onto supported cationic lipid bilayers which were imbedded in a matrix of negative surface potential, and the size of the cationic surface areas was varied from the micrometer down to the subnanometer length scale. The supported lipid bilayers and the adsorbed DNA were imaged with an atomic force microscope, and the DNA adsorption was found to depend on the surface charge density and on the size of the cationic lipid bilayers areas. While on purely cationic lipid bilayers larger than 20 nm a densely packed layer of DNA could be observed, as expected, no DNA could be detected on molecular cocrystals of cationic and anionic lipids where the diameter of the cationic lipid headgroups is only about 0.7 nm. On a 1:1 binary mixture of cationic and neutral lipids, on the other hand, the spacing between adjacent DNA strands was found to double, compared to the purely cationic lipid membranes.
引用
收藏
页码:8246 / 8251
页数:6
相关论文
共 46 条
[1]  
[Anonymous], 1998, ANGEW CHEM
[2]   Observation of a rectangular columnar phase in condensed lamellar cationic lipid-DNA complexes [J].
Artzner, F ;
Zantl, R ;
Rapp, G ;
Radler, JO .
PHYSICAL REVIEW LETTERS, 1998, 81 (22) :5015-5018
[3]   ATOMIC FORCE MICROSCOPE [J].
BINNIG, G ;
QUATE, CF ;
GERBER, C .
PHYSICAL REVIEW LETTERS, 1986, 56 (09) :930-933
[4]   PREDICTING DNA DUPLEX STABILITY FROM THE BASE SEQUENCE [J].
BRESLAUER, KJ ;
FRANK, R ;
BLOCKER, H ;
MARKY, LA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (11) :3746-3750
[5]   Long-range electrostatic interaction in DNA cationic lipid complexes [J].
Bruinsma, R ;
Mashl, J .
EUROPHYSICS LETTERS, 1998, 41 (02) :165-170
[6]   Electrostatics of DNA cationic lipid complexes: isoelectric instability [J].
Bruinsma, R .
EUROPEAN PHYSICAL JOURNAL B, 1998, 4 (01) :75-88
[7]  
CEVE G, 1993, PHOSPHOLIPIDS HDB
[8]  
de Gennes P.G., 1979, SCALING CONCEPTS POL
[9]   POLYMERS AT AN INTERFACE - A SIMPLIFIED VIEW [J].
DEGENNES, PG .
ADVANCES IN COLLOID AND INTERFACE SCIENCE, 1987, 27 (3-4) :189-209
[10]  
DOI M, 1996, INTRO POLYM PHYSICS