Metal enhanced fluorescence on nanoporous gold leaf-based assay platform for virus detection

被引：39

作者：

Ahmed, Syed Rahin ^{[1
,2
]}

Hossain, Md. Ashraf ^{[2
]}

Park, Jung Youn ^{[3
]}

Kim, Soo-Hyung ^{[2
]}

Lee, Dongyun ^{[4
]}

Suzuki, Tetsuro ^{[5
]}

Lee, Jaebeom ^{[4
]}

Park, Enoch Y. ^{[1
,6
]}

机构：

[1] Shizuoka Univ, Grad Sch Sci & Technol, Shizuoka 4228529, Japan

[2] Pusan Natl Univ, Dept Nano Fus Technol, Miryang 627706, South Korea

[3] Natl Fisheries Res & Dev Inst, Pusan 619705, South Korea

[4] Pusan Natl Univ, Dept Nano Fus Engn & Cognomechatron Engn, Pusan 609735, South Korea

[5] Hamamatsu Univ, Sch Med, Dept Infect Dis, Hamamatsu, Shizuoka 4313192, Japan

[6] Shizuoka Univ, Res Inst Green Sci & Technol, Shizuoka 4228529, Japan

来源：

BIOSENSORS & BIOELECTRONICS | 2014年 / 58卷

基金：

新加坡国家研究基金会;

关键词：

Nanoporous gold leaf; Surface roughness; Fluorescence enhancement; Quantum dot; Influenza A virus; EXCITON-PLASMON INTERACTIONS; INFLUENZA A/H1N1 2009; RAPID ANTIGEN TEST; CDTE NANOWIRES; NANOPARTICLES; INFECTION; DIAGNOSIS;

D O I：

10.1016/j.bios.2014.02.039

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In the present study, a rapid, sensitive and quantitative detection of influenza A virus targeting hemagglutinin (HA) was developed using hybrid structure of quantum dots (QDs) and nanoporous gold leaf (NPGL). NPGL film was prepared by dealloying bimetallic film where its surface morphology and roughness were fairly controlled. Anti-influenza A virus HA antibody (ab66189) was bound with NPGL and amine (-NH2) terminated QDs. These biofunctionalized NPGL and QDs formed a complex with the influenza virus A/Beijing/262/95 (H1N1) and the photoluminescence (PL) intensities of QDs were linearly correlated with the concentrations of the virus up to 1 ng/mL while no PL was observed in the absence of the virus, or in bovine serum albumin (BSA, 1 mu g/mL) alone. In addition, it was demonstrated that this assay detected successfully influenza virus A/Yokohama/110/2009 (H3N2) that is isolated from a clinical sample, at a concentration of ca. 50 plaque forming units (PFU)/mL. This detection limit is 2-order more sensitive than a commercially available rapid influenza diagnostic test. From these results, the proposed assay may offer a new strategy to monitor influenza virus for public health. (c) 2014 Elsevier B.V. All rights reserved.

引用

页码：33 / 39

页数：7

共 33 条

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