The characteristics of arginine transport by rat cerebellar and cortical synaptosomes

被引:16
作者
Aldridge, CR [1 ]
Collard, KJ [1 ]
机构
[1] UNIV WALES COLL CARDIFF,SCH MOL & MED BIOSCI,PHYSIOL UNIT,CARDIFF CF1 3US,S GLAM,WALES
关键词
L-arginine; uptake; synaptosomes; cerebellum; cortex; nitric oxide;
D O I
10.1007/BF02533103
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The uptake of L-[H-3]arginine into synaptosomes prepared from rat cerebellum and cortex occurred by a high-affinity carrier-mediated process. The uptake of arginine appeared to be potentiated by removal of extracellular Na+, inhibited by high levels of extracellular K+, but not by depolarization with veratridine or 4-amino pyridine. The effect of Na+ removal or K+ elevation did not seem to be due to changes in intracellular Ca2+ or pH. Ln both brain regions, uptake was significantly inhibited by L-arginine, L-lysine, L-ornithine, and L-homoarginine, but not by D-arginine nor L-citrulline. Uptake was also inhibited by N-G-monomethyl-L-arginine acetate, but not by N-G-nitro-L-arginine methyl ester nor N-G-nitro-L-arginine except in the cortex at a concentration of 1 mM. The results indicate that the carrier system operating in synaptosomes showed many of the characteristics of the ubiquitous y(+) system seen in many other tissues, although its apparent sensitivity to variations in extracellular Na+ was unusual.
引用
收藏
页码:1539 / 1546
页数:8
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