Identification of gene expression profile of dorsal root ganglion in the rat peripheral axotomy model of neuropathic pain

被引:407
作者
Xiao, HS
Huang, QH
Zhang, FX
Bao, L
Lu, YJ
Guo, C
Yang, L
Huang, WJ
Fu, G
Xu, SH
Cheng, XP
Yan, Q
Zhu, ZD
Zhang, X
Chen, Z
Han, ZG
Zhang, X
机构
[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Lab Sensory Syst, Shanghai 200031, Peoples R China
[2] Chinese Natl Human Genome Ctr, Shanghai 201203, Peoples R China
[3] Fourth Mil Med Univ, Inst Neurosci, Xian 710032, Peoples R China
关键词
D O I
10.1073/pnas.122231899
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Phenotypic modification of dorsal root ganglion (DRG) neurons represents an important mechanism underlying neuropathic pain. However, the nerve injury-induced molecular changes are not fully identified. To determine the molecular alterations in a broader way, we have carried out cDNA array on the genes mainly made from the cDNA libraries of lumbar DRGs of normal rats and of rats 14 days after peripheral axotomy. Of the 7,523 examined genes and expressed sequence tags (ESTs), the expression of 122 genes and 51 expressed sequence tags is strongly changed. These genes encompass a large number of members of distinct families, including neuropeptides, receptors, ion channels, signal transduction molecules, synaptic vesicle proteins, and others. Of particular interest is the up-regulation of gamma-aminobutyric acidA receptor alpha5 subunit, peripheral benzodiazepine receptor, nicotinic acetylcholine receptor alpha7 subunit, P2Y1 purinoceptor, Na+ channel beta2 subunit, and L-type Ca2+ channel alpha2delta-1 subunit. Our findings therefore reveal dynamic and complex changes in molecular diversity among DRG neurons after axotomy.
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收藏
页码:8360 / 8365
页数:6
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