In vitro characterization of the ORF1-ntrBC promoter of Rhizobium etli

被引：9

作者：

Martino, M ^{[1
]}

Riccio, A ^{[1
]}

Defez, R ^{[1
]}

Iaccarino, M ^{[1
]}

Patriarca, EJ ^{[1
]}

机构：

[1] CNR,INT INST GENET & BIOPHYS,I-80125 NAPLES,ITALY

来源：

FEBS LETTERS | 1996年 / 388卷 / 01期

关键词：

two-component system; ntr system; runoff transcription; gel retardation;

D O I：

10.1016/0014-5793(96)00507-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rhizobium sigma vegetative-dependent promoters are different from those of enteric bacteria and have never been characterized before, We report here the biochemical characterization of the ORF1-ntrBC promoter of Rhizobium etli, The minimal promoter region was located by means of a transcriptional fusion and further characterized by in vitro transcription and gel retardation experiments, Oligonucleotides used as DNA competitors in runoff transcription experiments allowed the precise localisation of the promoter region, Protein extracts from an ntrC(+), but not from an ntrC(-) strain, inhibited in vitro transcription, The NtrC protein was found to bind specifically to the promoter, where an NtrC binding site overlapping the transcription initiation site, is present.

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页码：53 / 58

页数：6

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