Bioenergy beads:: A tool for regeneration of ATP/NTP in biocatalytic synthesis

被引：27

作者：

Nahalka, Jozef

Gemeiner, Peter

Bucko, Marek

Wang, Peng George

机构：

[1] Slovak Acad Sci, Inst Chem, SK-84538 Bratislava, Slovakia

[2] Ohio State Univ, Dept Biochem, Columbus, OH 43210 USA

来源：

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2006年 / 34卷 / 05期

关键词：

ATP-regeneration; immobilization; inclusion bodies; polyphosphate kinase;

D O I：

10.1080/10731190600862886

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Active inclusion bodies of recombinant polyphosphate kinase were obtained by simple washing of Escherichia coli cells with nonionic detergent and then they were immobilized in agar/TiO2 beads. Bioenergy beads obtained are charged by polyphosphate to act as rechargeable suppply of adenosine/nucleoside triphosphates (ATP/NTP), a practical tool for synthesis of artificial receptors.

引用

页码：515 / 521

页数：7

共 17 条

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