Isolation of U(VI) reduction-deficient mutants of Shewanella putrefaciens

被引：42

作者：

Wade, R ^{[1
]}

DiChristina, TJ ^{[1
]}

机构：

[1] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 184卷 / 02期

关键词：

anaerobic respiration; uranium reduction; respiratory mutant screening technique; Shewanella putrefaciens;

D O I：

10.1111/j.1574-6968.2000.tb09005.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A U(VI) reduction-deficient mutant (Urr) screening technique was developed and combined with chemical mutagenesis procedures to identify a Urr mutant of Shewanella putrefaciens strain 200. The Urr mutant lacked the ability to grow anaerobically on U(VI) and NO;, yet retained the ability to grow anaerobically on eight other compounds as terminal electron acceptor. All 11 members of previously isolated sets of Fe(III) and Mn(IV) reduction-deficient mutants of S. putrefaciens 200 displayed Urr-positive phenotypes with the Urr screen and were capable of anaerobic growth on U(VI). This is the first reported isolation of a respiratory mutant that is unable to grow anaerobically on U(VI) as terminal electron acceptor. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：143 / 148

页数：6

共 32 条

[1] CONCENTRATION, OXIDATION-STATE, AND PARTICULATE FLUX OF URANIUM IN THE BLACK-SEA [J].