New multiplexed flow cytometric assay to measure anti-peptide antibody: a novel tool for monitoring immune responses to peptides used for immunization

被引：74

作者：

Komatsu, N

Shichijo, S

Nakagawa, M

Itoh, K

机构：

[1] Kurume Univ, Sch Med, Dept Immunol, Kurume, Fukuoka 8300011, Japan

[2] Kurume Univ, Sch Med,Dept Immunol, Tailor Made Vaccine Project,Japan Sci & Technol A, Preventure Program,Off Technol Transfer, Kurume, Fukuoka 830, Japan

来源：

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION | 2004年 / 64卷 / 06期

关键词：

antibody; Luminex (TM) system; peptide vaccination; screening;

D O I：

10.1080/00365510410007008

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Peptide-based vaccination has a great potential for the prevention and treatment of various diseases. There is, however, no appropriate monitoring system to measure immune responses to vaccinated peptides, which hampers the development of therapeutically effective vaccine regimens to various diseases. In this study a new multiplexed flow cytometric assay using the Luminex(TM) system to monitor humoral immune responses to vaccinated peptides is described. Although the sensitivity is mostly equal to that of the traditional enzyme-linked immunosorbent assay (ELISA), the new assay has several advantages over ELISA in that it minimizes the amount of sera needed, running costs and working periods, and thus will be a novel tool for monitoring immune responses to vaccinated peptides.

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收藏

页码：535 / 545

页数：11

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