Expression of miR-210 during erythroid differentiation and induction of γ-globin gene expression

被引:82
作者
Bianchi, Nicoletta [1 ]
Zuccato, Cristina [1 ]
Lampronti, Ilaria [1 ]
Borgatti, Monica [1 ]
Gambari, Roberto [1 ,2 ]
机构
[1] Univ Ferrara, Dept Biochem & Mol Biol, BioPharmaNet, I-44100 Ferrara, Italy
[2] Univ Ferrara, Ctr Biotechnol, I-44100 Ferrara, Italy
关键词
Erythroid differentiation; Globin genes; HbF; MicroRNA; beta-thalassemia; BETA-THALASSEMIA; MICRORNA EXPRESSION; PRECURSOR CELLS; K562; CELLS; HYPOXIA; ERYTHROPOIESIS; HEMATOPOIESIS; ACCUMULATION; MITHRAMYCIN; EPHRIN-A3;
D O I
10.5483/BMBRep.2009.42.8.493
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
MicroRNAs (mills) are a family of small noncoding RNAs that regulate gene expression by targeting mRNAs in a sequence specific manner, inducing translational repression or mRNA degradation. In this paper we have first analyzed by microarray the milt-profile in erythroid precursor cells from one normal and two thalassemic patients expressing different levels of fetal hemoglobin (one of them displaying HPFH phenotype). The microarray data were confirmed by RT-PCR analysis, and allowed us to identify miR-210 as an highly expressed mill in the erythroid precursor cells from the HPFH patient. When RT-PCR was performed on mithramycin-induced K562 cells and erythroid precursor cells, miR-210 was found to be induced in time-dependent and dose-dependent fashion, together with increased expression of the fetal gamma-globin genes. Altogether, the data suggest that miR-210 might be involved in increased expression of gamma-globin genes in differentiating erythroid cells. [BMB reports 2009; 42(8): 493-499]
引用
收藏
页码:493 / 499
页数:7
相关论文
共 33 条
[1]  
ALTER BP, 1979, EXP HEMATOL, V7, P200
[2]   MicroRNA functions in animal development and human disease [J].
Alvarez-Garcia, I ;
Miska, EA .
DEVELOPMENT, 2005, 132 (21) :4653-4662
[3]   Accumulation of γ-globin mRNA and induction of erythroid differentiation after treatment of human leukaemic K562 cells with tallimustine [J].
Bianchi, N ;
Chiarabelli, C ;
Borgatti, M ;
Mischiati, C ;
Fibach, E ;
Gambari, R .
BRITISH JOURNAL OF HAEMATOLOGY, 2001, 113 (04) :951-961
[4]   The DNA-binding drugs mithramycin and chromomycin are powerful inducers of erythroid differentiation of human K562 cells [J].
Bianchi, N ;
Osti, F ;
Rutigliano, C ;
Corradini, FG ;
Borsetti, E ;
Tomassetti, M ;
Mischiati, C ;
Feriotto, G ;
Gambari, R .
BRITISH JOURNAL OF HAEMATOLOGY, 1999, 104 (02) :258-265
[5]   Regulated expression of microRNAs in normal and polycythemia vera erythropoiesis [J].
Bruchova, Hana ;
Yoon, Donghoon ;
Agarwal, Archana M. ;
Mendell, Joshua ;
Prchal, Josef T. .
EXPERIMENTAL HEMATOLOGY, 2007, 35 (11) :1657-1667
[6]   hsa-miR-210 is induced by hypoxia and is an independent prognostic factor in breast cancer [J].
Camps, Carme ;
Buffa, Francesca M. ;
Colella, Stefano ;
Moore, John ;
Sotiriou, Christos ;
Sheldon, Helen ;
Harris, Adrian L. ;
Gleadle, Jonathan M. ;
Ragoussis, Jiannis .
CLINICAL CANCER RESEARCH, 2008, 14 (05) :1340-1348
[7]   MicroRNAs as regulators of mammalian hematopoiesis [J].
Chen, CZ ;
Lodish, HF .
SEMINARS IN IMMUNOLOGY, 2005, 17 (02) :155-165
[8]   MicroRNA expression profiling during human cord blood-derived CD34 cell erythropoiesis [J].
Choong, Meng Ling ;
Yang, Henry He ;
McNiece, Ian .
EXPERIMENTAL HEMATOLOGY, 2007, 35 (04) :551-564
[9]   Hypoxic regulation of miR-210: Shrinking targets expand HIF-1's influence [J].
Corn, Paul G. .
CANCER BIOLOGY & THERAPY, 2008, 7 (02) :265-267
[10]   The effect of hypoxia and stem cell source on haemoglobin switching [J].
Daisy Narayan, A ;
Ersek, A ;
Campbell, TA ;
Colón, DM ;
Pixley, JS ;
Zanjani, ED .
BRITISH JOURNAL OF HAEMATOLOGY, 2005, 128 (04) :562-570