A microfabricated fluorescence-activated cell sorter

被引：738

作者：

Fu, AY

Spence, C

Scherer, A

Arnold, FH

Quake, SR ^{[1
]}

机构：

[1] CALTECH, Dept Appl Phys, Pasadena, CA 91125 USA

[2] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

NATURE BIOTECHNOLOGY | 1999年 / 17卷 / 11期

基金：

美国国家科学基金会;

关键词：

microchips; cell sorting; disposable chip; flow cytometry;

D O I：

10.1038/15095

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have demonstrated a disposable microfabricated fluorescence-activated cell sorter (mu FACS) for sorting various biological entities. Compared with conventional FAGS machines, the mu FACS provides higher sensitivity, no cross-contamination, and lower cost. We have used mu FACS chips to obtain substantial enrichment of micron-sized fluorescent bead populations of differing colors. Furthermore, we have separated Escherichia coli cells expressing green fluorescent protein from a background of nonfluorescent E. coli cells and shown that the bacteria are viable after extraction from the sorting device. These sorters can function as stand-alone devices or as components of an integrated microanalytical chip.

引用

页码：1109 / 1111

页数：3

共 17 条

[1]

BRODY JP, 1998, Patent No. 656155

[2] Self-sorting of white blood cells in a lattice [J].