Biofilm matrix of Candida albicans and Candida tropicalis:: chemical composition and role in drug resistance

被引:392
作者
Al-Fattani, Mohammed A. [1 ]
Douglas, L. Julia [1 ]
机构
[1] Univ Glasgow, Inst Biomed & Life Sci, Div Infect & Immun, Glasgow G12 8QQ, Lanark, Scotland
关键词
D O I
10.1099/jmm.0.46569-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Matrix material was extracted from biofilms of Candida albicans and Candida tropicalis and analysed chemically. Both preparations contained carbohydrate, protein, hexosamine, phosphorus and uronic acid. However, the major component in C. albicans matrix was glucose (32 %), whereas in C. tropicalis matrix it was hexosamine (27 %). Biofilms of C. albicans were more easily detached from plastic surfaces by treatment with the enzyme lyticase (beta-1,3-glucanase) than were those of C, tropicalis. Biofilms of C. albicans were also partially detached by treatment with proteinase K, chitinase, DNase 1, or beta-N-acetylglucosaminidase, whereas C. tropicalis biofilms were only affected by lipase type VII or chitinase. To investigate a possible role for the matrix in biofilm resistance to antifungal agents, biofilms of C. albicans were grown under conditions of continuous flow in a modified Robbins device (MRD). These biofilms produced more matrix material than those grown statically, and were significantly more resistant to amphotericin B. Biofilms of C. tropicalis synthesized large amounts of matrix material even when grown statically, and such biofilms were completely resistant to both amphotericin B and fluconazole. Mixed-species biofilms of C. albicans and a slime-producing strain of Staphylococcus epidermidis (RP62A), when grown statically or in the MRD, were also completely resistant to amphotericin B and fluconazole. Mixed-species biofilms of C. albicans and a slime-negative mutant of S. epidermidis (W), on the other hand, were completely drug resistant only when grown under flow conditions. These results demonstrate that the matrix can make a significant contribution to drug resistance in Candida biofilms, especially underconditions similar to those found in catheter infections in vivo, and that the composition of the matrix material is an important determinant in resistance.
引用
收藏
页码:999 / 1008
页数:10
相关论文
共 44 条
[1]   Mixed species biofilms of Candida albicans and Staphylococcus epidermidis [J].
Adam, B ;
Baillie, GS ;
Douglas, LJ .
JOURNAL OF MEDICAL MICROBIOLOGY, 2002, 51 (04) :344-349
[2]   Penetration of Candida biofilms by antifungal agents [J].
Al-Fattani, MA ;
Douglas, LJ .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (09) :3291-3297
[3]   EFFECT OF POLYSACCHARIDE INTERACTIONS ON ANTIBIOTIC SUSCEPTIBILITY OF PSEUDOMONAS-AERUGINOSA [J].
ALLISON, DG ;
MATTHEWS, MJ .
JOURNAL OF APPLIED BACTERIOLOGY, 1992, 73 (06) :484-488
[4]   Methods to extract the exopolymeric matrix from biofilms: A comparative study [J].
Azeredo, J ;
Lazarova, V ;
Oliveira, R .
WATER SCIENCE AND TECHNOLOGY, 1999, 39 (07) :243-250
[5]   Matrix polymers of Candida biofilms and their possible role in biofilm resistance to antifungal agents [J].
Baillie, GS ;
Douglas, LJ .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2000, 46 (03) :397-403
[6]  
Baillie GS, 1999, METHOD ENZYMOL, V310, P644
[7]   Effect of growth rate on resistance of Candida albicans biofilms to antifungal agents [J].
Baillie, GS ;
Douglas, LJ .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (08) :1900-1905
[8]   Iron-limited biofilms of Candida albicans and their susceptibility to amphotericin B [J].
Baillie, GS ;
Douglas, LJ .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (08) :2146-2149
[9]   A MODIFIED URONIC ACID CARBAZOLE REACTION [J].
BITTER, T ;
MUIR, HM .
ANALYTICAL BIOCHEMISTRY, 1962, 4 (04) :330-&
[10]   ASSAY FOR TOTAL HEXOSAMINE AND A DIFFERENTIAL ASSAY FOR GLUCOSAMINE AND GALACTOSAMINE [J].
BLUMENKRANTZ, N ;
ASBOEHANSEN, G .
CLINICAL BIOCHEMISTRY, 1976, 9 (06) :269-274