Chromatographic characterization of a molecular imprinted polymer binding cortisol

被引：43

作者：

Baggiani, C

Giraudi, G

Trotta, F

Giovannoli, C

Vanni, A

机构：

[1] Univ Turin, Dipartimento Chim Analit, I-10125 Turin, Italy

[2] Univ Turin, Dipartimento Chim Inorgan Fis & Mat, I-10125 Turin, Italy

来源：

TALANTA | 2000年 / 51卷 / 01期

关键词：

cortisol; liquid chromatography; molecular imprinting; molecular recognition;

D O I：

10.1016/S0039-9140(99)00251-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A cortisol-binding polymer was prepared by utilising a non-covalent molecular imprinting polymerisation technique. The obtained polymer was packed in a high-performance liquid chromatography (HPLC) column; the selectivity was studied by liquid chromatography, eluting cortisol, cortisone, corticosterone, progesterone, 11-keto-progesterone, 11 alpha-hydroxyprogesterone, 17 alpha-hydroxyprogesterone, cortisol 21-hemisuccinate, and cortisol 21-acetate with chloroform, containing 0.5% (v/v) acetic acid, as mobile phase. The mechanism of molecular imprinting was confirmed and a good selectivity for cortisol, with limited recognition for cortisone and 11 alpha-hydroxyprogesterone, was found. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：71 / 75

页数：5

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