Comparison of FaRP immunoreactivity in free-living nematodes and in the plant-parasitic nematode Heterodera glycines

被引:11
作者
Masler, EP
Kovaleva, ES
Sardanelli, S
机构
[1] USDA ARS, Nematol Lab, Beltsville, MD 20705 USA
[2] Univ Maryland, Plant Nematol Lab, College Pk, MD 20742 USA
来源
NEUROPEPTIDES: STRUCTURE AND FUNCTION IN BIOLOGY AND BEHAVIOR | 1999年 / 897卷
关键词
D O I
10.1111/j.1749-6632.1999.tb07896.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The family of FMRFamide-related peptides (FaRPs) is widely distributed among invertebrates, where the peptides serve as neuromodulators, Published reports indicate that numerous FaRP sequences exist in free-living and animal parasitic nematodes, Using a FMRFamide ELISA, FaRP immunoreactivity was detected in extracts of the soybean cyst nematode, Heterodera glycines, in both sexes and at all developmental stages. HPLC-ELISA results revealed a number of immunoreactive components in H. glycines preparations, and a comparison with extracts of the free-living nematodes Caenorhabditis elegans and Panagrellus redivivus showed significant qualitative differences in FaRP immunoreactivity between the plant parasite and the two free-living nematodes, Total and specific immunoreactivities varied during H. glycines development, with the highest specific activity in juveniles and males, and the highest total activity in mature females. Total female immunoreactivity was located primarily within the mature eggs, A significant portion, however, was associated with the female body, perhaps with egg laying.
引用
收藏
页码:253 / 263
页数:11
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