Adipose stromal cells-conditional medium protected glutamate-induced CGNs neuronal death by BDNF

被引：56

作者：

Zhao, L. ^{[1
]}

Wei, X. ^{[1
]}

Ma, Z. ^{[1
,3
]}

Feng, D. ^{[2
]}

Tu, P. ^{[3
]}

Johnstone, B. H. ^{[2
]}

March, K. L. ^{[2
]}

Du, Y. ^{[1
]}

机构：

[1] Indiana Univ, Dept Neurol, Sch Med, Indianapolis, IN 46202 USA

[2] Indiana Univ, Sch Med, Dept Med, Indianapolis, IN 46202 USA

[3] Peking Univ, Sch Pharmaceut Sci, Beijing 100871, Peoples R China

来源：

NEUROSCIENCE LETTERS | 2009年 / 452卷 / 03期

关键词：

Excytotoxicity; Brain-derived neurotrophic factor; Adipose stromal cells; Neuroprotection; STEM-CELLS; APOPTOSIS;

D O I：

10.1016/j.neulet.2009.01.025

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

The delivery of factors secreted by adipose stromal cells (ASC) to the brain may be a viable neuroprotective therapeutic option. In this study, we investigated the bioactivity of ASC-conditioned medium (ASC-CM) in glutamate-induced neurotoxicity and found that the ASC-CM significantly blocked glutamate neurotoxicity. We identified the brain-derived neurotrophic factor (BDNF) in the ASC-CM by using Western blot and demonstrated that this activity was critical for the neuroprotective effect of ASC-CM in excytotoxicity models. Furthermore, inactivating BDNF also attenuated the suppression by ASC-CM of glutamate-induced caspase-3 activity, but not p38 phosphorylation. These findings suggest that among ASC secrete a potent combination of factors, BDNF play a major role in neuroprotection against excytotoxicity. (c) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：238 / 240

页数：3

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