Pyrene-labeled oligodeoxynucleotide probe for detecting base insertion by excimer fluorescence emission

被引：141

作者：

Okamoto, A

Ichiba, T

Saito, I ^{[1
]}

机构：

[1] Kyoto Univ, Fac Engn, Dept Synthet Chem & Biol Chem, Kyoto 6158510, Japan

[2] Japn Sci & Technol Corp, SORST, Kyoto 6158510, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 27期

关键词：

D O I：

10.1021/ja049061d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Novel ODN probes for insertion polymorphism detection have been devised. The ODN probes labeled with two pyrene chromophores emit strong excimer fluorescence when hybridized with DNA containing one inserted base. This method is useful for the determination of the presence/absence of insertion polymorphisms located at a specific site on the target DNA. Copyright © 2004 American Chemical Society.

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页码：8364 / 8365

页数：2

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