Effects of yeast elicitor on the growth and secondary metabolism of a high-tanshinone-producing line of the Ti transformed Salvia miltiorrhiza cells in suspension culture

被引：43

作者：

Hui, C ^{[1
]}

Feng, C ^{[1
]}

机构：

[1] Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China

来源：

PROCESS BIOCHEMISTRY | 2000年 / 35卷 / 08期

关键词：

Salvia miltiorrhiza; cryptotanshinone; rosmarinic acid; yeast elicitor;

D O I：

10.1016/S0032-9592(99)00146-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of yeast elicitor on the cell growth and secondary metabolism of a high-tanshinone-producing line (cell line B) of the Agrobacterium tumefaciens C58 transformed Salvia miltiorrhiza cells were investigated in suspension culture. Application of yeast elicitor resulted in an overall increase (up to ca. 100%) in cryptotanshinone production whereas the constituent level of rosmarinic acid was reduced (from 2.6% of dry weight to zero at 1.0-ml elicitor dosage). Yeast elicitation also led to a decrease in primary metabolism as indicated by a reduction in biomass accumulation. Differential strategies were suggested for the improvement of productivity of rosmarinic acid and cryptotanshinone in plant cell cultures. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：837 / 840

页数：4

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