Cross reactivity of polyclonal GFAP antiserum: implications for the in-vitro characterisation of brain endothelium

被引：4

作者：

Dolman, DEM

Lawrenson, JG

Abbott, NJ

机构：

[1] Kings Coll London, Neurosci Res Ctr, BBB Grp, London SE1 1UL, England

[2] City Univ London, Dept Optometry & Visual Sci, London EC1V 0HB, England

来源：

BRAIN RESEARCH | 2004年 / 1012卷 / 1-2期

基金：

英国惠康基金;

关键词：

cross reactivity; GFAP; in vitro characterization; endothelium;

D O I：

10.1016/j.brainres.2004.03.033

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

Following a recent claim, based on glial acidic fibrillary protein (GFAP) expression, that brain-derived astrocytes in culture are in fact endothelial cells, we immuno-labelled primary cultures of rat brain astrocytes and endothelium with various GFAP antisera. Both cell types stained positively with a polyclonal antibody, although monoclonal antiserum labelled only astrocytes. We conclude that staining of endothelial cells with the polyclonal GFAP antiserum is due to cross reactivity with another protein. (C) 2004 Elsevier B.V All rights reserved.

引用

页码：185 / 186

页数：2

共 4 条

[1]

ABBOTT NJ, 1992, J CELL SCI, V103, P23

[2]

Characteristics of endothelial cells derived from the blood-brain barrier and of astrocytes in culture [J].