Collagen but not fibrinogen surfaces induce bleb formation, exposure of phosphatidylserine, and procoagulant activity of adherent platelets: Evidence for regulation by protein tyrosine kinase-dependent Ca2+ responses

被引:215
作者
Heemskerk, JWM
Vuist, WMJ
Feijge, MAH
Reutelingsperger, CPM
Lindhout, T
机构
[1] UNIV MAASTRICHT,DEPT HUMAN BIOL,NL-6200 MD MAASTRICHT,NETHERLANDS
[2] UNIV MAASTRICHT,DEPT BIOCHEM,NL-6200 MD MAASTRICHT,NETHERLANDS
关键词
D O I
10.1182/blood.V90.7.2615.2615_2615_2625
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
With a combined phase-contrast and fluorescence video imaging system, changes in morphology and cytosolic [Ca2+](i) were investigated of fura-2-loaded platelets during adhesion to fibrinogen or collagen matrices. The Ca2+ signals were, on the level of single platelets, compared to the secretion and procoagulant responses, using fluorescent-labeled AK-6 antibody against P-selectin and labeled annexin V for detection of surface-exposed phosphatidylserine (PS), respectively. Platelets in contact with fibrinogen developed filapods and spread over the matrix, in most of the cells without detectable Ca2+ signal. Thrombin induced repetitive spiking in [Ca2+](i), followed by the expression of P-selectin but not of PS on the platelet surface. Platelet interaction with collagen resulted in spreading and transformation of the cells into blebbing, ''balloon''-like structures (diameter about 5 mu m). The latter morphological changes were accompanied by high and prolonged increases in [Ca2+](i), by the exposure of both P-selectin and PS, and by the ability of the platelets to convert prothrombin into thrombin. Thrombin addition accelerated the onset of the Ca2+ signals and the appearance of surface-exposed PS. Collagen-induced PS exposure was slightly reduced by treatment of the platelets with aspirin, and strongly inhibited by suppression of the Ca2+ responses with prostaglandin E-i or the Ca2+ chelator, dimethyl-BAPTA. Inhibition of protein tyrosine phosphorylation with genistein, U73343, or wortmannin resulted in spiking Ca2+ responses in many of the platelets and in almost complete reduction of bleb formation and PS exposure. In contrast, genistein did not suppress bleb formation and PS exposure of platelets stimulated with the Ca2+ ionophore A23187. We conclude that a collagen but not fibrinogen matrix acts as a potent activator of the procoagulant response through activation of tyrosine kinases and subsequent generation of sustained intracellular Ca2+ signals. (C) 1997 by The American Society of Hematology.
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页码:2615 / 2625
页数:11
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