Immunoprotective UVA (320-400 nm) irradiation upregulates heme oxygenase-1 in the dermis and epidermis of hairless mouse skin

被引：42

作者：

Allanson, M ^{[1
]}

Reeve, VE ^{[1
]}

机构：

[1] Univ Sydney, Fac Vet Sci, Sydney, NSW 2006, Australia

来源：

JOURNAL OF INVESTIGATIVE DERMATOLOGY | 2004年 / 122卷 / 04期

基金：

英国医学研究理事会;

关键词：

heme oxygenase; immunohistochemistry; in situ hybridization; mouse skin; UVA radiation;

D O I：

10.1111/j.0022-202X.2004.22421.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

The induction of heme oxygenase-1 (HO-1) by ultraviolet A (UVA) (320-400 nm) radiation provides a protective cellular defence against oxidative stress, and has been well demonstrated in cultured human skin fibroblasts, although keratinocytes were unreactive. The UVA responsiveness of HO-1 however, has not been confirmed in intact skin. Previously, we reported that UVA-inducible HO enzyme activity in mouse skin is protective against UVB-induced immunosuppression. This study identifies the induced HO isoform and its localization in mouse skin irradiated in vivo with such an immunoprotective UVA dose. We found that HO-1 mRNA was expressed in UVA-irradiated skin, but not in normal or UVB-irradiated skin, whereas constitutive HO-2 was always present. UVA-irradiated skin had increased HO enzyme activity and bilirubin content, and decreased heme content, consistent with HO-1 induction. In situ hybridization and immunohistochemical staining localized HO-1 mRNA and protein to both epidermis and dermis, with strongest expression in basal keratinocytes and weaker expression in dermal fibroblast-like and other cells, in contrast with UVA-induced HO-1 in cultured human skin fibroblasts. This suggests that cultured skin cells may not fully represent skin functions in vivo, or that there may be inherent differences between human and hairless mouse skin HO-1 responses.

引用

页码：1030 / 1036

页数：7

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