Specific and sensitive diagnosis of syphilis using a real-time PCR for Treponema pallidum

被引：45

作者：

Koek, A. G.

Bruisten, S. M.

Dierdorp, M.

van Dam, A. P.

Templeton, K.

机构：

[1] Amsterdam Municipal Hlth Lab, GGD, NL-1018 WT Amsterdam, Netherlands

[2] Dept Infect Dis, Amsterdam, Netherlands

[3] AMC, Dept Retrovirol, Amsterdam, Netherlands

[4] Leiden Univ, Med Ctr, Dept Med Microbiol, Leiden, Netherlands

[5] Royal Infirm Edinburgh NHS Trust, Specialist Virol Ctr, Edinburgh, Midlothian, Scotland

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2006年 / 12卷 / 12期

关键词：

diagnosis; genital samples; real-time PCR; sexually transmitted infections; syphilis; Treponema pallidum;

D O I：

10.1111/j.1469-0691.2006.01566.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A real-time PCR assay with a Taqman probe was developed that targeted the polA gene of Treponema pallidum. The test was validated using an analytical panel (n = 140) and a clinical panel of genital samples (n = 112) from patients attending a sexually transmitted infections clinic. High sensitivities and specificities of 94-100% were achieved using two real-time PCR platforms, the Rotor-Gene and the iCycler. The assay can be completed within 2 h, enabling reporting in <8 h. This fast and robust assay is suitable for implementation in routine laboratories for diagnosing primary syphilis.

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收藏

页码：1233 / 1236

页数：4

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