Application of isoelectric focusing in thin-layer polyacrylamide gels to the study of opine dehydrogenases in marine invertebrates

被引：6

作者：

Kanno, N ^{[1
]}

Kamimura, K ^{[1
]}

Nagahisa, E ^{[1
]}

Sato, M ^{[1
]}

Sato, Y ^{[1
]}

机构：

[1] TOHOKU UNIV,FAC AGR,SENDAI,MIYAGI 981,JAPAN

来源：

FISHERIES SCIENCE | 1996年 / 62卷 / 01期

关键词：

isoelectric focusing; opine dehydrogenase; distribution; substrate specificity; multiple enzyme forms; opine;

D O I：

10.2331/fishsci.62.122

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Application of isoelectric focusing in thin-layer polyacrylamide gels (IEF/PAG) to the study of opine dehydrogenases in marine invertebrates was described. The optimized IEF/PAG and the following activity bands detection showed high resolution and good reproducibility. It was useful for the detection of multi-functional enzymes which metabolize two (or more) distinct opines, and for the analysis of multiple enzyme forms (allozymes and isoenzymes) in tissues of marine invertebrates.

引用

页码：122 / 125

页数：4

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