Isolation, partial sequencing, and expression of pathogenesis-related cDNA genes from pepper leaves infected by Xanthomonas campestris pv. vesicatoria

被引:95
作者
Jung, HW [1 ]
Hwang, BK [1 ]
机构
[1] Korea Univ, Dept Agr Biol, Seoul 136701, South Korea
关键词
D O I
10.1094/MPMI.2000.13.1.136
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Specific cDNAs showing differential expression in bacteria-infected pepper leaves as opposed to healthy leaves were isolated from a pepper cDNA library from hypersensitive response (HR) lesions of leaves infected with an avirulent strain of Xanthomonas campestris pv, vesicatoria. Among a total of 282 cDNA clones tested, 36 individual cDNA genes (13%) hybridized strongly or differentially to the cDNA probes from bacteria-infected leaves. Ten Capsicum annuum-induced (CAI) genes encoding putative thionin,lipid transfer protein I and II, osmotin (PR-5), class I chitinase, beta-1,3-glucanase, SAR 8.2, stellacyanin, leucine-rich repeat protein, and auxin-repressed protein mere identified. Two CAI genes showed little or no sequence homology to the previously sequenced plant genes, Transcripts of the CAI genes were strongly or preferentially induced in pepper tissues by infection with X. campestris pv, vesicatoria or Phytophthora capsici, and by abiotic elicitor treatment, In particular, most of the CAI genes were strongly induced in pepper tissues by ethephon and methyl jasmonate.
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页码:136 / 142
页数:7
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