The effect of BisGMA on cyclooxygenase-2 expression, PGE2 production and cytotoxicity via reactive oxygen species- and MEK/ERK-dependent and -independent pathways

被引：65

作者：

Chang, Mei-Chi ^{[2
]}

Lin, Li-Deh ^{[1
]}

Chan, Chiu-Po ^{[3
]}

Chang, Hsiao-Hua ^{[1
]}

Chen, Lin-I ^{[1
,4
]}

Lin, Hsueh Jen ^{[1
,5
]}

Yeh, Hung-Wei ^{[1
]}

Tseng, Wan Yu ^{[1
]}

Lin, Po-Shuen ^{[1
]}

Lin, Chiu-Chun ^{[6
]}

Jeng, Jiiang-Huei ^{[1
]}

机构：

[1] Natl Taiwan Univ, Coll Med, Natl Taiwan Univ Hosp,Grad Inst Clin Dent, Lab Dent Pharmacol Toxicol & Mat Biocompatibil, Taipei 100, Taiwan

[2] Chang Gung Inst Technol, Biomed Sci Team, Tao Yuan, Taiwan

[3] Chang Gung Mem Hosp, Dept Dent, Taipei 10591, Taiwan

[4] Univ Adelaide, Dept Dent, Adelaide, SA 5005, Australia

[5] Show Chwan Mem Hosp, Dept Dent, Changhua, Taiwan

[6] Chang Gung Univ, Dept Dent, Chang Gung Mem Hosp, Kaohsiung, Taiwan

来源：

BIOMATERIALS | 2009年 / 30卷 / 25期

关键词：

BisGMA; Cytotoxicity; Human dental pulp cells; Prostaglandin; Reactive oxygen species; Signal transduction; CELL-CYCLE ARREST; PROSTAGLANDIN E-2 PRODUCTION; HUMAN GINGIVAL FIBROBLASTS; DENTAL-PULP CELLS; INTERLEUKIN-8; EXPRESSION; GLUTATHIONE DEPLETION; OXIDATIVE STRESS; EPITHELIAL-CELLS; RESIN MONOMERS; TEGDMA;

D O I：

10.1016/j.biomaterials.2009.04.034

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

After operative restoration, some monomers released from dentin bonding agents or composite resin may induce tissue inflammation and affect the vitality of dental pulp. Whether BisGMA, a major monomer of composite resin, may induce prostaglandin release and cytotoxicity to pulp cells and their mechanisms awaits investigation. We found that BisGMA induced cytotoxicity to human dental pulp cells at concentrations higher than 0.075 mm as analyzed by 3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. BisGMA (0.1 mM) also stimulated ERK phosphorylation, PGE(2) production, COX-2 mRNA and protein expression as well as ROS production (as indicated by an increase in cellular DCF fluorescence) in dental pulp cells. Catalase (500 and 1000 U/ml) and U0126 (10 and 20 mu M, a MEK inhibitor) effectively prevented the BisGMA-induced ERK activation, PGE(2) production and COX-2 expression. Moreover, catalase can protect the pulp cells from BisGMA cytotoxicity, whereas aspirin and U0126 lacked of this protective activity. These results suggest that BisGMA released from composite resin may potentially affect the vitality of dental pulp and induce pulpal inflammation via stimulation of ROS production, MEK/ERK1/2 activation and subsequent COX-2 gene expression and PGE(2) production. Cytotoxicity of BisGMA to dental pulp cells is related to ROS production, but not directly mediated by MEK activation and PGE(2) production. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：4070 / 4077

页数：8

共 37 条

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