An orthotopic colon cancer model for studying the B7-H3 antitumor effect in vivo

被引：63

作者：

Lupu, Catalin M.

Eisenbach, Christoph

Kuefner, Michael A.

Schmidt, Jan

Lupu, Alaviana D.

Stremmel, Wolfgang

Encke, Jens

机构：

[1] Univ Heidelberg, Dept Gastroenterol, D-69120 Heidelberg, Germany

[2] Univ Heidelberg, Dept Gen Surg, D-69120 Heidelberg, Germany

[3] Univ Heidelberg, Dept Haematol, D-69120 Heidelberg, Germany

来源：

JOURNAL OF GASTROINTESTINAL SURGERY | 2006年 / 10卷 / 05期

关键词：

animal model; colon cancer; costimulatory molecule;

D O I：

10.1016/j.gassur.2006.02.001

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

We established an orthotopic animal model of colon cancer in mice and applied this model to study the antitumor effects of B7-H3, the newest member of the B7 family of costimulatory molecules. Colon-26 murine colon adenocarcinoma cells were inoculated into the cecal subserosum of mice to induce colon tumor growth. The tumor growth rate and the survival time of the mice were observed. A stable B7-H3 transfected Colon-26 cell line was established and the immunogenic effect was investigated. All mice implanted with wild-type tumor cells had tumor growth in the colon and died. The mean survival rate was 23 days. Mice implanted with C26-B7-H3 had a significantly prolonged survival time of 38 days. Our data suggest that B7-H3 exerts an antitumor effect on adenocarcinoma of the colon and may be considered as an adjuvant immunotherapy in the treatment of colon cancers. Our orthotopic animal model of colon cancer in mice could be applied to in vivo experimental studies of colon cancer.

引用

页码：635 / 645

页数：11

共 31 条

[1]

Human-SCID mouse chimeric models for the evaluation of anti-cancer therapies [J].