Molecular characterization of the mouse vesicular acetylcholine transporter gene

被引:41
作者
Naciff, JM
Misawa, H
Dedman, JR
机构
[1] UNIV CINCINNATI, COLL MED, DEPT CELLULAR & MOL PHYSIOL, CINCINNATI, OH 45267 USA
[2] TOKYO METROPOLITAN INST NEUROSCI, DEPT NEUROL, FUCHU, TOKYO 183, JAPAN
关键词
5'-flanking region of the mouse choline acetyltransferase gene; molecular cloning; mouse; mouse cholinergic system; vesicular acetylcholine transporter gene;
D O I
10.1097/00001756-199711100-00011
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
THE organization of the mouse choline acetyltransferase (ChAT) gene has been previously analyzed. Here we show that the first intron of the mouse ChAT gene contains an uninterrupted open reading frame. It is in the same transcriptional orientation as ChAT and encodes the vesicular acetylcholine (ACh) transporter (VAChT), the protein responsible for the translocation of cytoplasmic ACh into synaptic vesicles. The sequence of this transporter is very similar to the VAChT from rat and human (99% and 95% identity, respectively). Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed expression of mouse VAChT mRNA in spinal cord, brain (excluding the cerebellum) and brain stem, but not in peripheral tissues such as liver and kidney. Transgenic mouse analysis revealed that the 5'-flanking region of the mouse ChAT gene encompasses regulatory elements that allowed elevated expression of VAChT in the cholinergic system of transgenic mice.
引用
收藏
页码:3467 / 3473
页数:7
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