Three tandem HRDC domains have synergistic effect on the RecQ functions in Deinococcus radiodurans

被引:30
作者
Huang, Lifen
Hua, Xiaoting
Lu, Huiming
Gao, Guanjun
Tian, Bing
Shen, Binghui
Hua, Yuejin [1 ]
机构
[1] Zhejiang Univ, Inst Nucl Agr Sci, Hangzhou 310029, Peoples R China
[2] Yangzhou Univ, Jiangsu Prov Key Lab Crop Genet & Physiol, Jiangsu 225009, Peoples R China
[3] City Hope Natl Med Ctr, Dept Radiat Biol, Duarte, CA 91010 USA
[4] Beckman Res Inst, Duarte, CA 91010 USA
基金
中国国家自然科学基金;
关键词
Deinococcus radiodurans; RecQ; helicase; HRDC;
D O I
10.1016/j.dnarep.2006.09.006
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The RecQ family of DNA helicases performs essential functions in the maintenance of genomic stability in all organisms. in Deinococcus radiodurans, DR1289 is a special member of RecQ family with unique arrangement of three tandem HRDC domains in the C-terminus. A dr1289 mutant is hypersensitive to gamma-irradiation, UV, H2O2 and mitomycin C. By complementing the dr1289 mutant with various domains of Dr1289 in vivo, we have determined that the helicase and all three HRDC domains are indispensable for complete DNA damage resistance. Using a continuous fluorescent dye-displacement assay, we investigated the optimal conditions for Dr1289 unwinding function at various concentrations of ATP and metal ions to show that the helicase activity is comparable to what observed in Escherichia coli RecQ. We also found that the helicase domain is necessary for the unwinding and ATPase activity and that the three tandem HRDC domains increase the efficiency of these activities. Based on these data, we propose that the C-terminus of Dr1289 has evolved in D. radiodurans to confront the types and amounts of DNA damage. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:167 / 176
页数:10
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