Simultaneous analysis of eight components in "Ping-Wei-San" by high performance liquid chromatography

A HPLC method for simultaneous determination of eight marker substances was established for the quality control of Chinese medicinal preparation "Ping-Wei-San." These substances were glycyrrhizin in Glycyrrhizae Radix; hesperidin, nobiletin, 3',4',3,5,6,7,8-heptamethoxyflavone, tangeretin, and 5-hydroxy-3',4',6,7,8-pentane-thoxyflavon in Citri Leiocarpae Exocarpium; honokiol and magnolol in Magnoliae Cortex. Extracted samples were separated on an Inertsil ODS-80A column at 30 degrees C with a mixture of 20% acetonitrile (pH 2.5) and 70% acetonitrile (pH 2.5) aqueous solution employing a linear gradient elution method at a flow-rate of 1.0 mL/min. The detection wavelength varied with time. It was 275 nm during 0 similar to 19 min and 250 nm during 19 similar to 80 min. Relative standard deviations of intra- and inter-day analysis were less than 5%. This separation method could be successfully applied for the simultaneous determination of eight marker substances in "Ping-Wei-San."