Simultaneous determination of clodronate and its partial ester derivatives by ion-pair reversed-phase high-performance liquid chromatography coupled with evaporative light-scattering detection

被引：39

作者：

Niemi, R ^{[1
]}

Taipale, H ^{[1
]}

Ahlmark, M ^{[1
]}

Vepsalainen, J ^{[1
]}

Jarvinen, T ^{[1
]}

机构：

[1] UNIV KUOPIO,DEPT CHEM,FIN-70211 KUOPIO,FINLAND

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 701卷 / 01期

基金：

芬兰科学院;

关键词：

clodronate;

D O I：

10.1016/S0378-4347(97)00342-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new ion-pair HPLC method coupled with evaporative light-scattering detection (ELSD) for the simultaneous determination of clodronate and its partial esters has been developed. The simultaneous chromatographic separation was achieved on a reversed-phase C-8 column with a gradient system and butylamine as an ion-pair reagent. This method provides good enough reproducibility and sensitivity for in vitro determinations of clodronate and its ester derivatives. The method is applied for hydrolysis studies of clodronate monoesters which have been described as possible prodrugs of clodronate. (C) 1997 Elsevier Science B.V.

引用

页码：97 / 102

页数：6

共 23 条

[1] COMPARATIVE MOLECULAR-FIELD ANALYSIS OF SOME CLODRONIC ACID-ESTERS [J].